Recombinant Full Length Human BCCIP Protein, C-Flag-tagged

• Cat.No.: BCCIP-344HFL
• Product Overview: Recombinant Full Length Human BCCIP Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene product was isolated on the basis of its interaction with BRCA2 and p21 proteins. It is an evolutionarily conserved nuclear protein with multiple interacting domains. The N-terminal half shares moderate homology with regions of calmodulin and M-calpain, suggesting that it may also bind calcium. Functional studies indicate that this protein may be an important cofactor for BRCA2 in tumor suppression, and a modulator of CDK2 kinase activity via p21. This protein has also been implicated in the regulation of BRCA2 and RAD51 nuclear focus formation, double-strand break-induced homologous recombination, and cell cycle progression. Multiple transcript variants encoding different isoforms have been described for this gene.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 35.8 kDa
• AA Sequence: MASRSKRRAVESGVPQPPDPPVQRD EEEEKEVENEDEDDDDSDKEKDEED EVIDEEVNIEFEAYSLSDND YDGIKKLLQQLFLKAPVNTAELTDL LIQQNHIGSVIKQTDVSEDSNDDMD EDEVFGFISLLNLTERKGTQ CVEQIQELVLRFCEKNCEKSMVEQL DKFLNDTTKPVGLLLSERFINVPPQ IALPMYQQLQKELAGAHRTN KPCGKCYFYLLISKTFVEAEKNNSK KKPSNKKKAALMFANAEEEFFYEKA ILKFNYSVQEESDTCLGGKW
  SFDDVPMTPLRTVMLIPGDKMNEIM DKLKEYLSVTRTRPLEQKLISEEDL AANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome, Stem cell - Pluripotency

Gene Information

• Gene Name: BCCIP BRCA2 and CDKN1A interacting protein [ Homo sapiens (human) ]
• Official Symbol: BCCIP
• Synonyms: TOK1; TOK-1
• Gene ID: 56647
• mRNA Refseq: NM_078468.3
• Protein Refseq: NP_510868.1
• MIM: 611883
• UniProt ID: Q9P287