Recombinant Full Length Human CA12 Protein, C-Flag-tagged
Cat.No. : | CA12-2169HFL |
Product Overview : | Recombinant Full Length Human CA12 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells. |
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Description : | Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. This gene product is a type I membrane protein that is highly expressed in normal tissues, such as kidney, colon and pancreas, and has been found to be overexpressed in 10% of clear cell renal carcinomas. Three transcript variants encoding different isoforms have been identified for this gene. |
Source : | Mammalian cells |
Species : | Human |
Tag : | Flag |
Form : | 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol. |
Molecular Mass : | 36.8 kDa |
AA Sequence : | MPRRSLHAAAVLLLVILKEQPSSPA PVNGSKWTYFGPDGENSWSKKYPSC GGLLQSPIDLHSDILQYDAS LTPLEFQGYNLSANKQFLLTNNGHS VKLNLPSDMHIQGLQSRYSATQLHL HWGNPNDPHGSEHTVSGQHF AAELHIVHYNSDLYPDASTASNKSE GLAVLAVLIEMGSFNPSYDKIFSHL QHVKYKGQEAFVPGFNIEEL LPERTAEYYRYRGSLTTPPCNPTVL WTVFRNPVQISQEQLLALETALYCT HMDDPSPREMINNFRQVQKF DERLVYTSFSQVQVCTAAGLSLGII LSLALAGILGICIVVVVSIWLFRRK SIKKGDNKGVIYKPATKMET EAHA TRTRPLEQKLISEEDLAANDILDYK DDDDKV |
Purity : | > 80% as determined by SDS-PAGE and Coomassie blue staining. |
Stability : | Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles. |
Storage : | Store at -80 centigrade. |
Concentration : | >50 ug/mL as determined by microplate BCA method. |
Preparation : | Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps. |
Protein Families : | Druggable Genome, Transmembrane |
Protein Pathways : | Nitrogen metabolism |
Gene Name : | CA12 carbonic anhydrase 12 [ Homo sapiens (human) ] |
Official Symbol : | CA12 |
Synonyms : | CAXII; CA-XII; T18816; HsT18816 |
Gene ID : | 771 |
mRNA Refseq : | NM_001218.5 |
Protein Refseq : | NP_001209.1 |
MIM : | 603263 |
UniProt ID : | O43570 |
Products Types
◆ Recombinant Protein | ||
Car12-1958M | Recombinant Mouse Car12 Protein, Myc/DDK-tagged | +Inquiry |
CA12-423R | Recombinant Rhesus Macaque CA12 Protein, His (Fc)-Avi-tagged | +Inquiry |
CA12-260M | Active Recombinant Mouse CA12 protein(Met1-Ser301), His-tagged | +Inquiry |
CA12-1456C | Recombinant Cynomolgus CA12 protein, His-tagged | +Inquiry |
CA12-2032H | Recombinant Human CA12 Protein, MYC/DDK-tagged | +Inquiry |
◆ Lysates | ||
CA12-3067MCL | Recombinant Mouse CA12 cell lysate | +Inquiry |
CA12-3061HCL | Recombinant Human CA12 cell lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (7)
Ask a questionKnown inhibitors can be identified through drug screening assays, and their impact on CA12 can be studied using kinetic studies and cell-based assays.
Cellular localization can be identified using immunofluorescence, subcellular fractionation, or electron microscopy.
CA12 has been implicated in tumor progression, particularly in hypoxic environments, by modulating pH and thus influencing tumor cell survival and metastasis. This can be assessed using cellular assays and tumor models.
Downstream pathways can be identified using pathway analysis tools, phosphoproteomics, and specific signal transduction assays.
The activity modulation under different pH conditions can be determined using enzymatic assays and pH shift experiments.
Expression changes in response to stressors can be analyzed using techniques like qPCR, Western blotting, or proteomics following cellular treatment with specific agents.
Genetic variants and their impact can be determined using structural models and functional assays comparing wild-type and mutant proteins.
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