Recombinant Full Length Human CA12 Protein, C-Flag-tagged

• Cat.No.: CA12-2169HFL
• Product Overview: Recombinant Full Length Human CA12 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. This gene product is a type I membrane protein that is highly expressed in normal tissues, such as kidney, colon and pancreas, and has been found to be overexpressed in 10% of clear cell renal carcinomas. Three transcript variants encoding different isoforms have been identified for this gene.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 36.8 kDa
• AA Sequence: MPRRSLHAAAVLLLVILKEQPSSPA PVNGSKWTYFGPDGENSWSKKYPSC GGLLQSPIDLHSDILQYDAS LTPLEFQGYNLSANKQFLLTNNGHS VKLNLPSDMHIQGLQSRYSATQLHL HWGNPNDPHGSEHTVSGQHF AAELHIVHYNSDLYPDASTASNKSE GLAVLAVLIEMGSFNPSYDKIFSHL QHVKYKGQEAFVPGFNIEEL LPERTAEYYRYRGSLTTPPCNPTVL WTVFRNPVQISQEQLLALETALYCT HMDDPSPREMINNFRQVQKF DERLVYTSFSQVQVCTAAGLSLGII LSLALAGILGICIVVVVSIWLFRRK SIKKGDNKGVIYKPATKMET EAHA TRTRPLEQKLISEEDLAANDILDYK DDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Nitrogen metabolism

Gene Information

• Gene Name: CA12 carbonic anhydrase 12 [ Homo sapiens (human) ]
• Official Symbol: CA12
• Synonyms: CAXII; CA-XII; T18816; HsT18816
• Gene ID: 771
• mRNA Refseq: NM_001218.5
• Protein Refseq: NP_001209.1
• MIM: 603263
• UniProt ID: O43570