Recombinant Human CA12 Protein, GST-Tagged

Cat.No. : CA12-0233H
Product Overview : Human CA12 full-length ORF (NP_001209.1, 1 a.a. - 354 a.a.) recombinant protein with GST-tag at N-terminal.
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Description : Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. This gene product is a type I membrane protein that is highly expressed in normal tissues, such as kidney, colon and pancreas, and has been found to be overexpressed in 10% of clear cell renal carcinomas. Three transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Jun 2014]
Source : Wheat Germ
Species : Human
Tag : GST
Molecular Mass : 65.9 kDa
AA Sequence : MPRRSLHAAAVLLLVILKEQPSSPA PVNGSKWTYFGPDGENSWSKKYPSC GGLLQSPIDLHSDILQYDASLTPLE FQGYNLSANKQFLLTNNGHSVKLNL PSDMHIQGLQSRYSATQLHLHWGNP NDPHGSEHTVSGQHFAAELHIVHYN SDLYPDASTASNKSEGLAVLAVLIE MGSFNPSYDKIFSHLQHVKYKGQEA FVPGFNIEELLPERTAEYYRYRGSL TTPPCNPTVLWTVFRNPVQISQEQL LALETALYCTHMDDPSPREMINNFR QVQKFDERLVYTSFSQVQVCTAAGL SLGIILSLALAGILGICIVVVVSIW LFRRKSIKKGDNKGVIYKPATKMET EAHA
Applications : Enzyme-linked Immunoabsorbent Assay
Western Blot (Recombinant protein)
Antibody Production
Protein Array
Notes : Best use within three months from the date of receipt of this protein.
Storage : Store at -80 centigrade. Aliquot to avoid repeated freezing and thawing.
Storage Buffer : 50 mM Tris-HCI, 10 mM reduced Glutathione, pH=8.0 in the elution buffer.
SDS-PAGE: 12.5% SDS-PAGE Stained with Coomassie Blue.
Gene Name : CA12 carbonic anhydrase XII [ Homo sapiens ]
Official Symbol : CA12
Synonyms : CA12; carbonic anhydrase XII; carbonic anhydrase 12; HsT18816; CA-XII; carbonic dehydratase; carbonate dehydratase XII; tumor antigen HOM-RCC-3.1.3; CAXII; FLJ20151;
Gene ID : 771
mRNA Refseq : NM_001218
Protein Refseq : NP_001209
MIM : 603263
UniProt ID : O43570

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (7)

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Are there any known inhibitors specific to CA12, and how do they impact its enzymatic function? 07/13/2021

Known inhibitors can be identified through drug screening assays, and their impact on CA12 can be studied using kinetic studies and cell-based assays.

In which specific cellular compartments is CA12 predominantly localized, and how does this localization influence its role? 06/12/2020

Cellular localization can be identified using immunofluorescence, subcellular fractionation, or electron microscopy.

What role does CA12 play in tumorigenesis, especially in hypoxic tumor environments? 08/29/2019

CA12 has been implicated in tumor progression, particularly in hypoxic environments, by modulating pH and thus influencing tumor cell survival and metastasis. This can be assessed using cellular assays and tumor models.

What downstream signaling pathways are activated or inhibited by CA12? 07/28/2019

Downstream pathways can be identified using pathway analysis tools, phosphoproteomics, and specific signal transduction assays.

How does CA12 activity get modulated under varying pH conditions? 04/08/2018

The activity modulation under different pH conditions can be determined using enzymatic assays and pH shift experiments.

How does CA12 expression vary in response to different cellular stressors or stimuli? 03/08/2018

Expression changes in response to stressors can be analyzed using techniques like qPCR, Western blotting, or proteomics following cellular treatment with specific agents.

How do genetic variants of the CA12 gene influence its protein structure and function? 10/18/2017

Genetic variants and their impact can be determined using structural models and functional assays comparing wild-type and mutant proteins.

Customer Reviews (3)

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Reviews
12/20/2022

    Professional assistance, invaluable for complex protein experiments.

    05/09/2021

      Cost-effective quantitation, ideal for budget-conscious labs.

      11/21/2020

        Impressive expertise, accelerates our research progress.

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