Recombinant Full Length Human MAPKAPK2 Protein, C-Flag-tagged

• Cat.No.: MAPKAPK2-1775HFL
• Product Overview: Recombinant Full Length Human MAPKAPK2 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene encodes a member of the Ser/Thr protein kinase family. This kinase is regulated through direct phosphorylation by p38 MAP kinase. In conjunction with p38 MAP kinase, this kinase is known to be involved in many cellular processes including stress and inflammatory responses, nuclear export, gene expression regulation and cell proliferation. Heat shock protein HSP27 was shown to be one of the substrates of this kinase in vivo. Two transcript variants encoding two different isoforms have been found for this gene.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 45.4 kDa
• AA Sequence: MLSNSQGQSPPVPFPAPAPPPQPPT PALPHPPAQPPPPPPQQFPQFHVKS GLQIKKNAIIDDYKVTSQVL GLGINGKVLQIFNKRTQEKFALKML QDCPKARREVELHWRASQCPHIVRI VDVYENLYAGRKCLLIVMEC LDGGELFSRIQDRGDQAFTEREASE IMKSIGEAIQYLHSINIAHRDVKPE NLLYTSKRPNAILKLTDFGF AKETTSHNSLTTPCYTPYYVAPEVL GPEKYDKSCDMWSLGVIMYILLCGY PPFYSNHGLAISPGMKTRIR MGQYEFPNPEWSEVSEEVKMLIRNL LKTEPTQRMTITEFMNHPWIMQSTK VPQTPLHTSRVLKEDKERWE DVKEEMTSALATMRVDYEQIKIKKI EDASNPLLLKRRKKARALEAAALAH TRTRPLEQKLISEEDLAANDILDYK DDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: MAPK signaling pathway, Neurotrophin signaling pathway, VEGF signaling pathway

Gene Information

• Gene Name: MAPKAPK2 MAPK activated protein kinase 2 [ Homo sapiens (human) ]
• Official Symbol: MAPKAPK2
• Synonyms: MK2; MK-2; MAPKAP-K2
• Gene ID: 9261
• mRNA Refseq: NM_032960.4
• Protein Refseq: NP_116584.2
• MIM: 602006
• UniProt ID: P49137