Active Recombinant Human MAPKAPK2, His-tagged

• Cat.No.: MAPKAPK2-1426H
• Product Overview: Recombinant human MAPKAPK2 (46-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal His tag.

Specification

• Description: MAPKAPK2 (MAPKAP kinase2) is a Ser/Thr protein kinase which is regulated via direct phosphorylation by p38 MAP kinase. In conjunction with p38 MAP kinase, MAPKAPK2 is known to be involved in many cellular processes including stress and inflammatory responses, nuclear export, gene expression regulation and cell proliferation. Heat shock protein HSP27 has been shown to be one of the substrates of MAPKAPK2, in vivo.
• Source: Sf9 Cells
• Species: Human
• Tag: His
• Form: Recombinant protein stored in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, 25% glycerol.
• Bio-activity: 254 nmol/min/mg
• Molecular Mass: ~41kDa
• Protein length: 46 aa-end
• Purity: >90%
• Applications: Kinase Assay, Western Blot
• Storage: Store at –70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. Avoid freeze/thaw cycles.
• Concentration: 0.1μg/μl

Gene Information

• Gene Name: MAPKAPK2 mitogen-activated protein kinase-activated protein kinase 2 [ Homo sapiens ]
• Official Symbol: MAPKAPK2
• Synonyms: MAPKAPK2; mitogen-activated protein kinase-activated protein kinase 2; MAP kinase-activated protein kinase 2; MAPKAP kinase 2; MAPK-activated protein kinase 2; MK2; MK-2; MAPKAP-K2
• Gene ID: 9261
• mRNA Refseq: NM_004759
• Protein Refseq: NP_004750
• MIM: 602006
• UniProt ID: P49137
• Chromosome Location: 1q32
• Pathway: Activated TLR4 signalling, organism-specific biosystem; B Cell Receptor Signaling Pathway, organism-specific biosystem; CREB phosphorylation, organism-specific biosystem; Destabilization of mRNA by Butyrate Response Factor 1 (BRF1), organism-specific biosystem; Destabilization of mRNA by Tristetraprolin (TTP), organism-specific biosystem; FAS pathway and Stress induction of HSP regulation, organism-specific biosystem; Gene Expression, organism-specific biosystem
• Function: ATP binding; nucleotide binding; protein binding; protein kinase activity; protein serine/threonine kinase activity; protein serine/threonine kinase activity; signal transducer activity

Reviews

04/15/2022

The lab product sets a new standard for quality. Delivery was speedy, and the customer service team was exceptionally helpful.

11/23/2020

Reliable product, friendly customer service, and efficient delivery.

08/06/2019

Precision in quality, responsive customer service, and fast, secure delivery

Q&As

What structural features of MAPKAPK2 are critical for its kinase activity, and how do these features influence its interactions with substrates and regulatory partners?

MAPKAPK2's kinase activity is influenced by its structural features, including the catalytic domain. These features determine substrate interactions and regulatory partnerships, providing insights into its molecular functions.

What is known about the role of MAPKAPK2 in the immune response, particularly in the regulation of cytokine production and immune cell functions?

MAPKAPK2 plays a role in the immune response by regulating cytokine production and immune cell functions. It influences the activity of transcription factors involved in immune-related gene expression.

How does MAPKAPK2 contribute to cellular motility, and what molecular mechanisms are involved in its regulation of the cytoskeleton and cell migration?

MAPKAPK2 regulates cellular motility by influencing the cytoskeleton and cell migration. It phosphorylates proteins involved in cytoskeletal dynamics, impacting cell motility and invasive behavior.

Can you elaborate on the regulatory mechanisms controlling MAPKAPK2 activation and its substrate specificity?

MAPKAPK2 activation is regulated by p38 MAP kinase through phosphorylation. Its substrate specificity is determined by specific phosphorylation motifs, and its activity is influenced by interacting proteins.

In cancer research, how is MAPKAPK2 implicated in tumor development and progression, and are there potential therapeutic strategies targeting this kinase?

MAPKAPK2 has been associated with tumor development and progression. Understanding its role in cancer biology may lead to the development of targeted therapies, although challenges exist in achieving specificity.