Recombinant Full Length Human ME1 Protein, C-Flag-tagged

• Cat.No.: ME1-782HFL
• Product Overview: Recombinant Full Length Human ME1 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene encodes a cytosolic, NADP-dependent enzyme that generates NADPH for fatty acid biosynthesis. The activity of this enzyme, the reversible oxidative decarboxylation of malate, links the glycolytic and citric acid cycles. The regulation of expression for this gene is complex. Increased expression can result from elevated levels of thyroid hormones or by higher proportions of carbohydrates in the diet.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 64 kDa
• AA Sequence: MEPEAPRRRHTHQRGYLLTRNPHLN KDLAFTLEERQQLNIHGLLPPSFNS QEIQVLRVVKNFEHLNSDFD RYLLLMDLQDRNEKLFYRVLTSDIE KFMPIVYTPTVGLACQQYSLVFRKP RGLFITIHDRGHIASVLNAW PEDVIKAIVVTDGERILGLGDLGCN GMGIPVGKLALYTACGGMNPQECLP VILDVGTENEELLKDPLYIG LRQRRVRGSEYDDFLDEFMEAVSSK YGMNCLIQFEDFANVNAFRLLNKYR NQYCTFNDDIQGTASVAVAG LLAALRITKNKLSDQTILFQGAGEA ALGIAHLIVMALEKEGLPKEKAIKK IWLVDSKGLIVKGRASLTQE KEKFAHEHEEMKNLEAIVQEIKPTA LIGVAAIGGAFSEQILKDMAAFNER PIIFALSNPTSKAECSAEQC YKITKGRAIFASGSPFDPVTLPNGQ TLYPGQGNNSYVFPGVALGVVACGL RQITDNIFLTTAEVIAQQVS DKHLEEGRLYPPLNTIRDVSLKIAE KIVKDAYQEKTATVYPEPQNKEAFV RSQMYSTDYDQILPDCYSWP
  EEVQKIQTKVDQTRTRPLEQKLISE EDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Pathways: Metabolic pathways, PPAR signaling pathway, Pyruvate metabolism

Gene Information

• Gene Name: ME1 malic enzyme 1 [ Homo sapiens (human) ]
• Official Symbol: ME1
• Synonyms: MES; HUMNDME
• Gene ID: 4199
• mRNA Refseq: NM_002395.6
• Protein Refseq: NP_002386.1
• MIM: 154250
• UniProt ID: P48163