| Description : |
Enables cytokine activity and growth factor activity. Acts upstream of or within several processes, including animal organ development; generation of neurons; and positive regulation of macromolecule metabolic process. Located in extracellular space. Is expressed in several structures, including alimentary system; central nervous system; cranium; early conceptus; and female reproductive system. Orthologous to human LIF (LIF interleukin 6 family cytokine). |
| Source : |
E. coli |
| Species : |
Human |
| Tag : |
C-6×His |
| Molecular Mass : |
65.2 KD |
| AA Sequence : |
MEPEAPRRRHTHQRGYLLTRNPHLN KDLAFTLEERQQLNIHGLLPPSFNS QEIQVLRVVKNFEHLNSDFDRYLLL MDLQDRNEKLFYRVLTSDIEKFMPI VYTPTVGLACQQYSLVFRKPRGLFI TIHDRGHIASVLNAWPEDVIKAIVV TDGERILGLGDLGCNGMGIPVGKLA LYTACGGMNPQECLPVILDVGTENE ELLKDPLYIGLRQRRVRGSEYDDFL DEFMEAVSSKYGMNCLIQFEDFANV NAFRLLNKYRNQYCTFNDDIQGTAS VAVAGLLAALRITKNKLSDQTILFQ GAGEAALGIAHLIVMALEKEGLPKE KAIKKIWLVDSKGLIVKGRASLTQE KEKFAHEHEEMKNLEAIVQEIKPTA LIGVAAIGGAFSEQILKDMAAFNER PIIFALSNPTSKAECSAEQCYKITK GRAIFASGSPFDPVTLPNGQTLYPG QGNNSYVFPGVALGVVACGLRQITD NIFLTTAEVIAQQVSDKHLEEGRLY PPLNTIRDVSLKIAEKIVKDAYQEK TATVYPEPQNKEAFVRSQMYSTDYD QILPDCYSWPEEVQKIQTKVDQLEH HHHHH |
| Purity : |
> 95% by SDS-PAGE |
| Usage : |
Protein binding assay, small molecule inhibitor screening, substrate of kinase, antigen, ELISA, Western blot, crystallization and co- crystallization studies. |
| Storage : |
Upon delivery aliquot and store at -80 centigrade. Avoid multiple freeze-thaw cycles. The protein is stable for 12 months at -80 centigrade, for 2-4 weeks at 4 centigrade. |
| Storage Buffer : |
30 mM Tris pH 7.4; 500 mM NaCl; 10% glycerol; 3 mM MgCl2; 5 mM beta-mercaptoethanol; 1 mM PMSF; 1 mM Benzamidine |
| Reconstitution : |
Clear solution in 30 mM Tris, pH7.4, 150 mM NaCl, 40% Glycerol, 5 mM DTT |
