Recombinant Human ACBD4 293 Cell Lysate
- Gene Information
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|Description :||Antigen standard for acyl-Coenzyme A binding domain containing 4 (ACBD4), transcript variant 2 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.|
|Source :||HEK 293 cells|
|Components :||This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).|
|Size :||0.1 mg|
|Storage Instruction :||Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.|
|Applications :||ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.|
|Gene Name :||ACBD4 acyl-CoA binding domain containing 4 [ Homo sapiens ]|
|Official Symbol :||ACBD4|
|Synonyms :||ACBD4; acyl-CoA binding domain containing 4; acyl Coenzyme A binding domain containing 4; acyl-CoA-binding domain-containing protein 4; FLJ13322; acyl-Coenzyme A binding domain containing 4; HMFT0700; FLJ90623;|
|Gene ID :||79777|
|mRNA Refseq :||NM_001135704|
|Protein Refseq :||NP_001129176|
|UniProt ID :||Q8NC06|
|Chromosome Location :||17q21.31|
|Function :||binding; fatty-acyl-CoA binding;|
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
Q&As (10)Ask a question
Studies have shown that ACBD4 expression is altered in metabolic diseases such as obesity and diabetes. ACBD4 knockout mice exhibit reduced body weight, improved glucose tolerance, and reduced hepatic steatosis, suggesting that ACBD4 may be a potential target for the treatment of metabolic diseases.
It is generally stable when stored at -20°C or -80°C for up to six months to two years, respectively. However, the protein may be sensitive to freeze-thaw cycles, and repeated freeze-thaw cycles should be avoided. It is also recommended to aliquot the protein upon arrival to minimize the number of freeze-thaw cycles.
Yes, it can be used in a variety of functional assays, such as enzyme assays, binding assays, and activity assays, to study its biochemical and biophysical properties.
It may vary depending on the application and the specific experimental conditions. Typically, a concentration of 0.1-1 mg/mL is sufficient for most assays, but this may need to be optimized based on the experimental setup. It is recommended to use a range of concentrations to determine the optimal concentration for a specific experiment.
It varies depending on the specific application. It is important to verify the appropriate concentration for each specific application in order to ensure optimal results.
The endotoxin concentration is typically below 1.0 eu/μg of protein, as determined by a Limulus Amebocyte Lysate (LAL) assay. In addition, you can choose the concentration you need when submitting the inquiry form.
You can describe your experimental needs in detail to our scientists, such as WB, IF, IP, IHC, and they will provide professional solutions.
It should be stored at -20°C or -80°C in a non-frost free freezer. It is important to avoid repeated freeze-thaw cycles in order to prevent degradation of the protein. It is recommended that the protein be aliquoted into smaller portions to avoid contamination from repeated use.
ACBD4 protein contains an N-terminal acyl-CoA binding domain that binds to long-chain acyl-CoA esters and a C-terminal domain that interacts with the Golgi membrane. It also contains a hydrophobic loop that plays a crucial role in the binding of ACBD4 to the Golgi membrane.
Several signaling pathways, including the AMP-activated protein kinase (AMPK) and the mammalian target of rapamycin (mTOR) pathways, have been shown to regulate ACBD4 expression.
Customer Reviews (3)Write a review
I saved a lot of time and effort by purchasing the recombinant human ACBD4 protein from Creative BioMart instead of making it myself. -
The recombinant mouse ACBD4 protein from Creative BioMart was reasonably priced and saved me money compared to other suppliers. -
I am very satisfied with the quality and performance of the Recombinant Zebrafish ACBD4. It met all of our research needs. -
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