Recombinant Human ATP2B3 Protein, His-tagged
|Product Overview :||Recombinant Human ATP2B3 Protein (Thr1057-Leu1220) with N-His tag was expressed in E. coli.|
- Gene Information
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|Description :||The protein encoded by this gene belongs to the family of P-type primary ion transport ATPases characterized by the formation of an aspartyl phosphate intermediate during the reaction cycle. These enzymes remove bivalent calcium ions from eukaryotic cells against very large concentration gradients and play a critical role in intracellular calcium homeostasis. The mammalian plasma membrane calcium ATPase isoforms are encoded by at least four separate genes and the diversity of these enzymes is further increased by alternative splicing of transcripts. The expression of different isoforms and splice variants is regulated in a developmental, tissue- and cell type-specific manner, suggesting that these pumps are functionally adapted to the physiological needs of particular cells and tissues. This gene encodes the plasma membrane calcium ATPase isoform 3. Alternatively spliced transcript variants encoding different isoforms have been identified.|
|Source :||E. coli|
|Form :||Freeze-dried powder|
|Molecular Mass :||Predicted Molecular Mass: 22.1 kDa
Accurate Molecular Mass: 25 kDa
|Protein length :||Thr1057-Leu1220|
|Purity :||> 90%|
|Applications :||Positive Control; Immunogen; SDS-PAGE; WB.|
|Stability :||The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.|
|Storage :||Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.|
|Storage Buffer :||PBS, pH7.4, containing 0.01% SKL, 1 mM DTT, 5% Trehalose and Proclin300.|
|Reconstitution :||Reconstitute in sterile water to a concentration of 0.1-1.0 mg/mL. Do not vortex.|
|Gene Name :||ATP2B3 ATPase, Ca++ transporting, plasma membrane 3 [ Homo sapiens (human) ]|
|Official Symbol :||ATP2B3|
|Synonyms :||ATP2B3; ATPase, Ca++ transporting, plasma membrane 3; plasma membrane calcium-transporting ATPase 3; plasma membrane calcium transporting ATPase 3; PMCA3; plasma membrane calcium pump; plasma membrane calcium ATPase; PMCA3a|
|Gene ID :||492|
|mRNA Refseq :||NM_001001344|
|Protein Refseq :||NP_001001344|
|UniProt ID :||Q16720|
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
Q&As (6)Ask a question
There are several differences in protein structure and function between ATP2B3 and other atpase B family members, mainly depending on how they are expressed and regulated in different tissues and cells.
ATP2B3 gene mutation may be associated with some hereditary diseases such as pigmentary papillomatosis and atrial fibrillation/flutte
At present, studies have shown that ATP2B3 is associated with nervous system diseases such as Parkinson's disease and Alzheimer's disease.
The production pathway of ATP2B3 protein mainly includes multiple biological processes such as gene transcription, RNA splicing, protein translation and subsequent modification.
The regulatory mechanism of ATP2B3 may involve multiple signaling pathways and factors, such as calcium ions, ATP, membrane receptors, etc.
The relationship between ATP2B3 and cardiovascular disease may be achieved through several mechanisms such as regulating cardiomyocyte contraction and vascular smooth muscle cell contraction.
Customer Reviews (3)Write a review
The structural and subcellular localization information of the recombinant ATP2B3 protein can be evaluated by structural biology techniques and cellular immunostaining, among other methods. -
Purity and stability: ATP2B3 recombinant proteins are usually subjected to rigorous purification steps to ensure high purity protein samples. In addition, proper storage conditions and buffers can ensure the stability of the protein, thereby maintaining its activity. -
Activity and function: The activity of ATP2B3 recombinant protein is usually assessed by measuring its Ca2 + -pumping activity in vitro. ATP2B3 protein is a calcium pump, which is responsible for pumping calcium out of the cytoplasm in the cell. This is essential for cellular Ca 2 + homeostasis and signaling. -
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