Recombinant Human AXL protein, His-tagged

• Cat.No.: AXL-770H
• Product Overview: Recombinant Human AXL protein (Ala26-Pro449), fused to His tag at C-terminus, was expressed in human 293 cells (HEK293).

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: 424
• Description: AXL Receptor Tyrosine Kinase is also known as Tyrosine-protein kinase receptor UFO, which belongs to the protein kinase superfamily, Tyr protein kinase family and AXL/UFO subfamily. AXL contains two fibronectin type-III domains, two Ig-like C2-type (immunoglobulin-like) domains and one protein kinase domain. AXL is highly expressed in metastatic colon tumors. AXL is activated by GAS6-binding and subsequent autophosphorylation. AXL is involved in signal transduction from the extracellular matrix into the cytoplasm by binding growth factors, and thus implicated in the stimulation of cell proliferation.
• Form: Lyophilized from 0.22 um filtered solution in PBS, pH7.4, 10% trehalose.
• Molecular Mass: The protein has a calculated MW of 46.6 kDa. The protein migrates as 60-70 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Endotoxin: Less than 1.0 EU per ug by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
  Please avoid repeated freeze-thaw cycles.
  This product is stable after storage at:
  -20 centigrade to -70 centigrade for 12 months in lyophilized state;
  -70 centigrade for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: AXL
• Official Symbol: AXL
• Synonyms: AXL; AXL receptor tyrosine kinase; tyrosine-protein kinase receptor UFO; JTK11; UFO; AXL oncogene; oncogene AXL; AXL transforming sequence/gene
• Gene ID: 558
• mRNA Refseq: NM_001699
• Protein Refseq: NP_001690
• MIM: 109135
• UniProt ID: P30530

Citation

Conversion of RNA Aptamer into Modified DNA Aptamers Provides for Prolonged Stability and Enhanced Antitumor Activity.

Journal: Journal of the American Chemical Society    PubMed ID: 33988982    Data: 2021/12/15

Authors: Paola Amero, Ganesh L R Lokesh, Gabriel Lopez-Berestein

Article Snippet:Microscale Thermophoresis (MST).Microscale Thermophoresis (MST).. Microscale thermophoresis (MST) binding experiments were carried out with 5 nM Cy5-labeled aptamers (GLB-G25, GLB-A04) and 100 nM Cy3 labeled scrambled control aptamer in binding buffer (1x PBS pH 7.4, 0.1% Triton X 100) with a range of concentrations of AXL protein (Creative BioMart, Shirley, NY) from 0118 to 3781 nM at 40% MST power, 30% LED power (Cy5 aptamers), or 80% LED power (Cy3 aptamer) in standard capillaries on a Monolith NT.115 pico device (Cy5 aptamers) or Monolith NT115 (Cy3 aptamer) at 25 °C (NanoTemper Technologies, Munich, Germany).. Data was analyzed using MO.Data was analyzed using MO.