Recombinant Human CA12 protein, His-tagged
Cat.No. : | CA12-7846H |
Product Overview : | Recombinant Human CA12 aa. (Ala25~Leu302 (Accession # O43570)) fused with N-terminal His tag was produced in E. coli cells. |
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Description : | Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. This gene product is a type I membrane protein that is highly expressed in normal tissues, such as kidney, colon and pancreas, and has been found to be overexpressed in 10% of clear cell renal carcinomas. Three transcript variants encoding different isoforms have been identified for this gene. |
Source : | E. coli |
Species : | Human |
Tag : | His |
Form : | Freeze-dried powder |
Molecular Mass : | Predicted Molecular Mass: 32.8kDa |
Protein length : | Ala25~Leu302 (Accession # O43570) |
Endotoxin : | <1.0EU per 1ug (determined by the LAL method) |
Purity : | >95% |
Characteristic : | The isoelectric point is 6. |
Applications : | SDS-PAGE; WB; ELISA; IP |
Stability : | The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition. |
Storage : | Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months. |
Storage Buffer : | Supplied as lyophilized form in PBS, pH7.4, containing 1mM DTT, 5% trehalose, 0.05% sarcosyl and preservative. |
Reconstitution : | Reconstitute in sterile PBS, pH7.2-pH7.4. |
Gene Name : | CA12 carbonic anhydrase 12 [ Homo sapiens (human) ] |
Official Symbol : | CA12 |
Synonyms : | CA12; carbonic anhydrase 12; CAXII; CA-XII; T18816; HsT18816; carbonate dehydratase XII; carbonic anhydrase XII; carbonic dehydratase; tumor antigen HOM-RCC-3.1.3 |
Gene ID : | 771 |
mRNA Refseq : | NM_001218.4 |
Protein Refseq : | NP_001209.1 |
UniProt ID : | O43570 |
Products Types
◆ Recombinant Protein | ||
CA12-0233H | Recombinant Human CA12 Protein, GST-Tagged | +Inquiry |
CA12-260M | Active Recombinant Mouse CA12 protein(Met1-Ser301), His-tagged | +Inquiry |
CA12-1456C | Recombinant Cynomolgus CA12 protein, His-tagged | +Inquiry |
CA12-478H | Recombinant Human CA12 Protein, His (Fc)-Avi-tagged | +Inquiry |
CA12-261H | Recombinant Human CA12 protein(Met1-Gln291), His-tagged | +Inquiry |
◆ Lysates | ||
CA12-3061HCL | Recombinant Human CA12 cell lysate | +Inquiry |
CA12-3067MCL | Recombinant Mouse CA12 cell lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Write a reviewProfessional assistance, invaluable for complex protein experiments.
Cost-effective quantitation, ideal for budget-conscious labs.
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Q&As (7)
Ask a questionKnown inhibitors can be identified through drug screening assays, and their impact on CA12 can be studied using kinetic studies and cell-based assays.
Cellular localization can be identified using immunofluorescence, subcellular fractionation, or electron microscopy.
CA12 has been implicated in tumor progression, particularly in hypoxic environments, by modulating pH and thus influencing tumor cell survival and metastasis. This can be assessed using cellular assays and tumor models.
Downstream pathways can be identified using pathway analysis tools, phosphoproteomics, and specific signal transduction assays.
The activity modulation under different pH conditions can be determined using enzymatic assays and pH shift experiments.
Expression changes in response to stressors can be analyzed using techniques like qPCR, Western blotting, or proteomics following cellular treatment with specific agents.
Genetic variants and their impact can be determined using structural models and functional assays comparing wild-type and mutant proteins.
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