Recombinant Human CA5A Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: CA5A-2562H
• Product Overview: CA5A MS Standard C13 and N15-labeled recombinant protein (NP_001730) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. CA VA is localized in the mitochondria and expressed primarily in the liver. It may play an important role in ureagenesis and gluconeogenesis. CA5A gene maps to chromosome 16q24.3 and an unprocessed pseudogene has been assigned to 16p12-p11.2.
• Source: HEK293
• Species: Human
• Tag: Myc&DDK
• Molecular Mass: 34.75 kDa
• AA Sequence: MLGRNTWKTSAFSFLVEQMWAPLWS RSMRPGRWCSQRSCAWQTSNNTLHP LWTVPVSVPGGTRQSPINIQWRDSV YDPQLKPLRVSYEAASCLYIWNTGY LFQVEFDDATEASGISGGPLENHYR LKQFHFHWGAVNEGGSEHTVDGHAY PAELHLVHWNSVKYQNYKEAVVGEN GLAVIGVFLKLGAHHQTLQRLVDIL PEIKHKDARAAMRPFDPSTLLPTCW DYWTYAGSLTTPPLTESVTWIIQKE PVEVAPSQLSAFRTLLFSALGEEEK MMVNNYRPLQPLMNRKVWASFQATN EGTRSSGPTRTRRLEQKLISEEDLA ANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: CA5A carbonic anhydrase 5A [ Homo sapiens (human) ]
• Official Symbol: CA5A
• Synonyms: CA5A; carbonic anhydrase VA, mitochondrial; CA5; carbonic anhydrase 5A, mitochondrial; CAV; CAVA; CA-VA; carbonic dehydratase; carbonate dehydratase VA; carbonic anhydrase V, mitochondrial
• Gene ID: 763
• mRNA Refseq: NM_001739
• Protein Refseq: NP_001730
• MIM: 114761
• UniProt ID: P35218

Reviews

08/26/2022

Efficient protein quantification, saves time and resources.

01/15/2022

Consistency in results, a symbol of their service quality.

05/25/2021

Top-tier protein sequencing, propels our research forward.

Q&As

What post-translational modifications are observed in CA5A?

Post-translational modifications of CA5A can be identified using mass spectrometry, with potential phosphorylation or acetylation sites.

What diseases or conditions are associated with CA5A dysregulation?

Dysregulation of CA5A might influence metabolic processes in the liver, potentially impacting conditions like fatty liver disease or other metabolic disorders.

Are there genetic mutations in the CA5A gene that impact its function?

Specific mutations might affect CA5A's stability or activity, necessitating functional assays for validation.

Are there specific compounds known to inhibit or activate CA5A activity?

While general carbonic anhydrase inhibitors might affect CA5A, specific modulators would need further research.

What is the primary function of CA5A in cellular processes?

CA5A facilitates the reversible conversion of CO2 to bicarbonate and protons, playing a role in pH regulation and CO2 transport.

How does CA5A's enzymatic activity compare with other carbonic anhydrase isoforms?

CA5A's activity is tailored to its mitochondrial environment, which might differ in kinetics from cytosolic isoforms.

How does the expression profile of CA5A differ among human tissues?

CA5A is primarily expressed in the mitochondria of liver cells, with lesser amounts in other tissues.