Recombinant Human CASP3 protein, GST-tagged

• Cat.No.: CASP3-334H
• Product Overview: Recombinant Human CASP3(1 a.a. - 277 a.a.) fused with GST tag at N-terminal was expressed in Wheat Germ.

Specification

• Description: This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 6, 7 and 9, and the protein itself is processed by caspases 8, 9 and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. Alternative splicing of this gene results in two transcript variants that encode the same protein.
• Source: Wheat Germ
• Species: Human
• Tag: GST
• Form: 50 mM Tris-HCI, 10 mM reduced Glutathione, pH=8.0 in the elution buffer.
• Molecular Mass: 56.21 kDa
• AA Sequence: MENTENSVDSKSIKNLEPKIIHGSE SMDSGISLDNSYKMDYPEMGLCIII NNKNFHKSTGMTSRSGTDVDAANLR ETFRNLKYEVRNKNDLTREEIVELM RDVSKEDHSKRSSFVCVLLSHGEEG IIFGTNGPVDLKKITNFFRGDRCRS LTGKPKLFIIQACRGTELDCGIETD SGVDDDMACHKIPVEADFLYAYSTA PGYYSWRNSKDGSWFIQSLCAMLKQ YADKLEFMHILTRVNRKVATEFESF SFDATFHAKKQIPCIVSILTKELYF YH
• Applications: Enzyme-linked Immunoabsorbent Assay; Western Blot (Recombinant protein); Antibody Production; Protein Array
• Notes: Best use within three months from the date of receipt of this protein.
• Storage: Store at -80 centigrade. Aliquot to avoid repeated freezing and thawing.

Gene Information

• Gene Name: CASP3 caspase 3, apoptosis-related cysteine peptidase [ Homo sapiens ]
• Official Symbol: CASP3
• Synonyms: CASP3; caspase 3, apoptosis-related cysteine peptidase; caspase 3, apoptosis related cysteine protease; caspase-3; apopain; CPP32; CPP32B; Yama; CASP-3; CPP-32; procaspase3; protein Yama; PARP cleavage protease; cysteine protease CPP32; SREBP cleavage activity 1; caspase 3, apoptosis-related cysteine protease; SCA-1
• Gene ID: 836
• mRNA Refseq: NM_004346
• Protein Refseq: NP_004337
• MIM: 600636
• UniProt ID: P42574
• Chromosome Location: 4q34
• Pathway: Activation of DNA fragmentation factor, organism-specific biosystem; Activation of caspases through apoptosome-mediated cleavage, organism-specific biosystem; Alpha6-Beta4 Integrin Signaling Pathway, organism-specific biosystem; Alzheimers disease, organism-specific biosystem; Alzheimers disease, conserved biosystem; Amoebiasis, organism-specific biosystem; Amoebiasis, conserved biosystem
• Function: aspartic-type endopeptidase activity; cyclin-dependent protein kinase inhibitor activity; cysteine-type endopeptidase activity; cysteine-type endopeptidase activity; peptidase activity; protein binding