Recombinant Human CASP3 293 Cell Lysate

Cat.No. : CASP3-7839HCL
  • Specification
  • Gene Information
  • Related Products
  • Download
Description : Antigen standard for caspase 3, apoptosis-related cysteine peptidase (CASP3), transcript variant beta is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source : HEK 293 cells
Species : Human
Components : This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size : 0.1 mg
Storage Instruction : Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications : ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Tag : Non
Gene Name : CASP3 caspase 3, apoptosis-related cysteine peptidase [ Homo sapiens ]
Official Symbol : CASP3
Synonyms : CASP3; caspase 3, apoptosis-related cysteine peptidase; caspase 3, apoptosis related cysteine protease; caspase-3; apopain; CPP32; CPP32B; Yama; CASP-3; CPP-32; procaspase3; protein Yama; PARP cleavage protease; cysteine protease CPP32; SREBP cleavage activity 1; caspase 3, apoptosis-related cysteine protease; SCA-1;
Gene ID : 836
mRNA Refseq : NM_032991
Protein Refseq : NP_116786
MIM : 600636
UniProt ID : P42574
Chromosome Location : 4q34
Pathway : Activation of DNA fragmentation factor, organism-specific biosystem; Activation of caspases through apoptosome-mediated cleavage, organism-specific biosystem; Alpha6-Beta4 Integrin Signaling Pathway, organism-specific biosystem; Alzheimers disease, organism-specific biosystem; Alzheimers disease, conserved biosystem; Amoebiasis, organism-specific biosystem; Amoebiasis, conserved biosystem;
Function : aspartic-type endopeptidase activity; cyclin-dependent protein kinase inhibitor activity; cysteine-type endopeptidase activity; cysteine-type endopeptidase activity; peptidase activity; protein binding;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

Inquiry

  • Reviews
  • Q&As

Customer Reviews (1)

Write a review
Reviews
03/29/2024

    Casp3-748R, the rat caspase3 worked/is working very well for us.

    Q&As (0)

    Ask a question

    Ask a Question for All CASP3 Products

    Required fields are marked with *

    My Review for All CASP3 Products

    Required fields are marked with *

    0

    Inquiry Basket

    cartIcon
    logo

    FOLLOW US

    Terms and Conditions        Privacy Policy

    Copyright © 2024 Creative BioMart. All Rights Reserved.

    Contact Us

    • /
    • Service lnquiry:

    Stay Updated on the Latest Bioscience Trends