Recombinant Human CASP3 293 Cell Lysate
Cat.No. : | CASP3-7839HCL |
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Description : | Antigen standard for caspase 3, apoptosis-related cysteine peptidase (CASP3), transcript variant beta is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection. |
Source : | HEK 293 cells |
Species : | Human |
Components : | This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol). |
Size : | 0.1 mg |
Storage Instruction : | Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment. |
Applications : | ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane. |
Tag : | Non |
Gene Name : | CASP3 caspase 3, apoptosis-related cysteine peptidase [ Homo sapiens ] |
Official Symbol : | CASP3 |
Synonyms : | CASP3; caspase 3, apoptosis-related cysteine peptidase; caspase 3, apoptosis related cysteine protease; caspase-3; apopain; CPP32; CPP32B; Yama; CASP-3; CPP-32; procaspase3; protein Yama; PARP cleavage protease; cysteine protease CPP32; SREBP cleavage activity 1; caspase 3, apoptosis-related cysteine protease; SCA-1; |
Gene ID : | 836 |
mRNA Refseq : | NM_032991 |
Protein Refseq : | NP_116786 |
MIM : | 600636 |
UniProt ID : | P42574 |
Chromosome Location : | 4q34 |
Pathway : | Activation of DNA fragmentation factor, organism-specific biosystem; Activation of caspases through apoptosome-mediated cleavage, organism-specific biosystem; Alpha6-Beta4 Integrin Signaling Pathway, organism-specific biosystem; Alzheimers disease, organism-specific biosystem; Alzheimers disease, conserved biosystem; Amoebiasis, organism-specific biosystem; Amoebiasis, conserved biosystem; |
Function : | aspartic-type endopeptidase activity; cyclin-dependent protein kinase inhibitor activity; cysteine-type endopeptidase activity; cysteine-type endopeptidase activity; peptidase activity; protein binding; |
Products Types
◆ Recombinant Protein | ||
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CASP3-803R | Recombinant Rat CASP3 Protein, His (Fc)-Avi-tagged | +Inquiry |
CASP3-188H | Recombinant Human CASP3 Protein, MYC/DDK-tagged, C13 and N15-labeled | +Inquiry |
CASP3-623C | Recombinant Cattle CASP3 Protein, His-tagged | +Inquiry |
CASP3-0594H | Recombinant Human CASP3 Protein (M1-H277), Tag Free | +Inquiry |
◆ Lysates | ||
CASP3-7838HCL | Recombinant Human CASP3 293 Cell Lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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03/29/2024
Casp3-748R, the rat caspase3 worked/is working very well for us.
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