| Description : |
This gene is located in a cluster of similar genes in the same region of chromosome 17. These genes encode CC cytokines, which are secreted proteins characterized by two adjacent cysteines. The product of this gene is chemotactic for T cells and monocytes, and acts through C-C chemokine receptor type 1 (CCR1). The proprotein is further processed into numerous smaller functional peptides. Naturally-occurring readthrough transcripts occur from this gene into the downstream gene, CCL14 (chemokine (C-C motif) ligand 14). |
| Source : |
E.coli |
| Species : |
Human |
| Form : |
Lyophilized from a 0.2 μm filtered concentrated solution in 1 × PBS, pH 7.2. |
| Bio-activity : |
Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human T-lymphocytes is in a concentration range of 1.0-10 ng/ml. |
| Molecular Mass : |
Approximately 7.4 kDa, a single non-glycosylated polypeptide chain containing 68 amino acids. |
| Protein length : |
68 |
| AA Sequence : |
SFHFAADCCTSYISQSIPCSLMKSY FETSSECSKPGVIFLTKKGRQVCAK PSGPGVQDCMKKLKPYSI |
| Endotoxin : |
Less than 0.1 EU/μg of rHuMIP-5, 68a.a./CCL15 as determined by LAL method. |
| Purity : |
>98% by SDS-PAGE and HPLC analyses. |
| Storage : |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 centigrade as supplied. 1 month, 2 to 8 centigrade under sterile conditions after reconstitution. 3 months, -20 to -70 centigrade under sterile conditions after reconstitution. |
| Reconstitution : |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 centigrade. Further dilutions should be made in appropriate buffered solutions. |
