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Recombinant Human CD70 protein, His-tagged

Cat.No.: CD70-56H
Product Overview: Recombinant human CD70, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
Description: CD70, also known as CD70 antigen, is a transmembrane protein in the TNF receptor superfamily. It functions as a co-stimulatory molecule that supports lymphocyte activation and survival. This protein binds to the transmembrane glycoprotein CD27 ligand/CD70 which is expressed on activated B cells, activated T cells, and dendritic cells. Binding of CD70 to its receptor CD27 induces in priming, effector functions, differentiation and memory formation of T cell activation, and thus is proliferation of co-stimulated T cells, as well as the generation of cytolytic T cells.
Source: Insect cells
Species: Human
Tag: His tag
Form: Liquid. In Phosphate Buffered Saline (pH 7.4) containing 10% glycerol.
Molecular Mass: 18.2kDa (164aa)
AA Sequence: ADPQRFAQAQ QQLPLESLGW DVAELQLNHT GPQQDPRLYW QGGPALGRSF LHGPELDKGQ LRIHRDGIYM VHIQVTLAIC SSTTASRHHP TTLAVGICSP ASRSISLLRL SFHQGCTIAS QRLTPLARGD TLCTNLTGTL LPSRNTDETF FGVQWVRPHH HHHH
Endotoxin: < 1.0 EU per 1 microgram of protein (determined by LAL method)
Purity: >90% by SDS - PAGE
Storage: Can be stored at +4centigrade short term (1-2 weeks). For long term storage, aliquot and store at -20Centigrade or -70Centigrade. Avoid repeated freezing and thawing cycles.
Concentration: 0.25mg/ml (determined by Absorbance at 280nm)
Gene Name: CD70 CD70 molecule [ Homo sapiens ]
Official Symbol: CD70
Synonyms: CD70; CD70 molecule; CD70 antigen; CD27L; CD27-L; CD27LG; TNFSF7
Gene ID: 970
mRNA Refseq: NM_001252
Protein Refseq: NP_001243
MIM: 602840
UniProt ID: P32970
Chromosome Location: 19p13
Pathway: Cytokine-cytokine receptor interaction, organism-specific biosystem; Cytokine-cytokine receptor interaction, conserved biosystem;
Function: cytokine activity; protease binding; protein binding; receptor binding; tumor necrosis factor receptor binding;

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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