Recombinant Human CD70 cell lysate
Cat.No. : | CD70-2710HCL |
Product Overview : | Human CD70 / CD27L / TNFSF7 derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls |
- Specification
- Gene Information
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Source : | Human cells |
Species : | Human |
Preparation method : | Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. |
Lysis buffer : | Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF |
Quality control Testing : | 12.5% SDS-PAGE Stained with Coomassie Blue |
Recommended Usage : | 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating. |
Stability : | Samples are stable for up to twelve months from date of receipt at -80°C |
Storage Buffer : | 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF |
Storage Instruction : | Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes. |
Gene Name : | CD70 CD70 molecule [ Homo sapiens ] |
Official Symbol : | CD70 |
Synonyms : | CD70; CD70 molecule; CD27LG, TNFSF7, tumor necrosis factor (ligand) superfamily, member 7; CD70 antigen; CD27L; CD27-L; CD27 ligand; Ki-24 antigen; surface antigen CD70; tumor necrosis factor ligand superfamily member 7; tumor necrosis factor (ligand) superfamily, member 7; CD27LG; TNFSF7; |
Gene ID : | 970 |
mRNA Refseq : | NM_001252 |
Protein Refseq : | NP_001243 |
MIM : | 602840 |
UniProt ID : | P32970 |
Chromosome Location : | 19p13 |
Pathway : | Cytokine-cytokine receptor interaction, organism-specific biosystem; Cytokine-cytokine receptor interaction, conserved biosystem; |
Function : | cytokine activity; protease binding; protein binding; receptor binding; tumor necrosis factor receptor binding; |
Products Types
◆ Lysates | ||
CD70-1303RCL | Recombinant Rat CD70 cell lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (5)
Ask a questionAntibodies, antibody-drug conjugates, and chimeric antigen receptor (CAR) T-cell therapies are being developed to target CD70 in cancer.
CD70-targeted therapies can enhance the body's immune response against cancer cells and potentially lead to more effective treatments.
CD70 expression is often observed in Hodgkin's lymphoma and certain types of T-cell lymphomas.
Challenges include potential toxicities and finding the right balance to target CD70 effectively without harming normal immune functions.
Yes, there are ongoing clinical trials exploring CD70-targeted therapies for various cancer types.
Customer Reviews (3)
Write a reviewTheir collaboration, expertise, and customized solutions contribute to the successful execution of experiments, reliable data generation, and the advancement of scientific knowledge in the field.
the manufacturer's support is crucial for researchers using CD70 protein in trials.
These resources can enhance the efficiency and accuracy of experimental workflows and contribute to successful outcomes.
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