Recombinant Human Chemokine (C-C motif) Ligand 21

• Cat.No.: CCL21-341H
• Product Overview: Recombinant human CCL21is a single, non-glycosylated polypeptide chain containing 111 amino acids.CCL21 is expressed in E. coli, MW 12.2 kDa.

Specification

• Cat. No.: CCL21-341H
• Description: Chemokine (C-Cmotif) ligand 21 (CCL21) is a small cytokine belonging to the CC chemokinefamily. This chemokine is also known as 6Ckine (because it has six conservedcysteine residues instead of the four cysteines typical to chemokines),exodus-2, and secondary lymphoid-tissue chemokine (SLC). The gene for CCL21is located on human chromosome 9. CCL21 elicits its effects by binding to acell surface chemokine receptor known as CCR7.
• Source: Human
• Host: E. coli
• Form: Lyophilizedfrom a sterile filtered solution in 20 mM PB, pH 7.4 + 150 mM NaCl.
• Purity: > 97% asdetermined by HPLC and SDS-PAGE analyses.
• Sequence: SDGGAQDCCLKYSQRKIPAK VVRSYRKQEP SLGCSIPAIL FLPRKRSQAE LCADPKELWV QQLMQHLDKT PSPQKPAQGCRKDRGASKTG KKGKGSKGCR KTERSQTPKG P
• Molecular Weight: 12.2 kDa
• Biological Activity: Calculatedby the ability to chemoattract lymphocytes using a concentration of10.0-100.0ng/ml.
• Endotoxin Level: < 0.1ng/µg Exodus 2
• Reconstitution: Centrifugevial prior to opening. Add sterile distilled water or aqueous buffer containing0.1% BSA to a concentration of 0.1-1.0 mg/mL. Please note that the additionof any carrier protein into this product may produce unwanted endotoxin. Thisdepends upon the particular application employed. Further dilutions should bemade in appropriate buffered solutions.
• Storage: Stableat 2-8°C, but best kept desiccated -20°C. Upon reconstitution, stable for upto 1 week at 2-8°C. For longer term, store in working aliquots below -20°C. Avoidrepeated freeze/thaw cycles.
• OfficialSymbol: CCL21

Gene Information

• Gene Name: CCL21chemokine (C-C motif) ligand 21 [ Homo sapiens ]
• Synonyms: CCL21; chemokine (C-C motif) ligand 21; ECL; SLC; CKb9; TCA4;6Ckine; SCYA21; MGC34555; C-C motif chemokine 21; exodus-2;OTTHUMP00000021303; OTTHUMP00000021304; beta chemokine exodus-2;beta-chemokine exodus-2; small-inducible cytokine A21; secondary lymphoidtissue chemokine; secondary lymphoid-tissue chemokine; EfficientChemoattractant for Lymphocytes; small inducible cytokine subfamily A(Cys-Cys), member 21
• Gene ID: 6366
• mRNA Refseq: NM_002989
• Protein Refseq: NP_002980
• MIM: 602737
• UniProt ID: O00585
• Chromosome Location: 9p13
• Pathway: Chemokinereceptors bind chemokines; Chemokine signaling pathway; Class A/1(Rhodopsin-like receptors); Cytokine-cytokine receptor interaction; G alpha(i) signalling events; GPCR downstream signaling; GPCR ligand binding;Peptide ligand-binding receptors; Signaling by GPCR
• Function: CCR7chemokine receptor binding; chemokine activity