Recombinant Cynomolgus CCL21 cell lysate

• Cat.No.: CCL21-424CCL
• Product Overview: Cynomolgus CCL21 / SCYA21 derived in Baculovirus-Insect cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls

Specification

• Source: Baculovirus-Insect Cells
• Species: Cynomolgus
• Preparation method: Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
• Lysis buffer: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
• Quality control Testing: 12.5% SDS-PAGE Stained with Coomassie Blue
• Recommended Usage: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
• Stability: Samples are stable for up to twelve months from date of receipt at -80°C
• Storage Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
• Storage Instruction: Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
• Tag: Non

Gene Information

• Gene Name: CCL21 chemokine (C-C motif) ligand 21 [ Macaca fascicularis ]
• Official Symbol: CCL21
• Synonyms: chemokine (C-C motif) ligand 21; C-C chemokine ligand 21
• Gene ID: 102135349
• mRNA Refseq: NM_001283460
• Protein Refseq: NP_001270389
• Chromosome Location: chromosome: 15
• Pathway: Chemokine signaling pathway, organism-specific biosystem; Chemokine signaling pathway, conserved biosystem; Cytokine-cytokine receptor interaction, organism-specific biosystem

Reviews

11/21/2022

CCL21 products are reliable and stable, and are widely used in scientific research, industrial production and medical fields.

04/10/2022

CCL21 has a long half-life and can maintain stability and reliability during the experiment.

06/28/2021

When using CCL21 protein for experiments, it can meet specific experimental requirements and research purposes.

Q&As

Does CCL21 have relationships with other genes or proteins?

This has a complex relationship with other genes or proteins, and can interact with other genes or proteins to participate in a variety of biochemical reactions.

What are the applications of CCL21 in medical treatment?

It can be used as a biomarker in medical treatment to help monitor disease progression and treatment efficacy. In addition, therapeutics targeting CCL21, such as targeted therapies or modulating its expression, are also being investigated.

What diseases can CCL21 deficiency or aberrant expression cause?

CCL21 deficiency or aberrant expression may be associated with some autoimmune diseases, cancer, and neurodegenerative diseases.

Might abnormal CCL21 levels be indicative of disease?

Abnormal levels of CCL21 may suggest autoimmune diseases, cancer, and neurodegenerative diseases, but they may also be associated with other diseases.

How to study the regulatory mechanism of CCL21?

Studying the regulatory mechanism of CCL21 requires a combination of various experimental methods and techniques, such as gene knockout, transcriptome analysis, and protein-protein interactions.

How are mutations in CCL21 detected and analyzed?

Mutations in CCL21 can be detected and analyzed by methods such as whole-genome sequencing or target region sequencing to understand the impact of mutations on protein structure and function.