CCL21

  • Official Full Name

    chemokine (C-C motif) ligand 21

  • Overview

    Recombinant Mouse Chemokine (C-C Motif) Ligand 21A (Serine)
  • Synonyms

    SLC protein, Exodus 2, Exodus-2 protein, 6Ckine protein, Exodus-2 protein, Exodus 2, Exodus 2 protein, Exodus-2, CCL21 protein;

  • Recombinant Proteins
  • Cell & Tissue Lysates
  • Protein Pre-coupled Magnetic Beads
  • Cynomolgus Monkey
  • Human
  • Mouse
  • Rat
  • Rhesus Macaque
  • CHO
  • E.coli
  • HEK293
  • Human
  • Human Cells
  • In Vitro Cell Free System
  • Insect Cell
  • Insect Cells
  • Mammalian Cell
  • Mammalian cells
  • Wheat Germ
  • Biotin
  • DYKDDDDK
  • Fc
  • GST
  • His
  • His (Fc)
  • Avi
  • His|GST
  • N/A
  • N
Species Cat.# Product name Source (Host) Tag Protein Length Price
Human CCL21-122H Active Recombinant Human Chemokine (C-C Motif) Ligand 21, MIgG2a Fc-tagged CHO Fc
Human CCL21-626H Active Recombinant Human CCL21 protein(Met1-Pro134) Insect Cells N/A Met1-Pro134
Human CCL21-124H Active Recombinant Human Chemokine (C-C Motif) Ligand 21, HIgG1 Fc-tagged CHO Fc
Human CCL21-123H Active Recombinant Human Chemokine (C-C Motif) Ligand 21, HIgG1 Fc-tagged, mutant CHO Fc
Human CCL21-522H Active Recombinant Human CCL21 Mammalian cells N/A
Human CCL-329H Recombinant Human Chemokine (C-C Motif) Ligand 21, His-tagged E.coli His
Human CCL21-341H Recombinant Human Chemokine (C-C motif) Ligand 21 Human N/A
Human CCL21-10843H Recombinant Human CCL21, GST-tagged E.coli GST C-term-115a.a.
Human CCL21-0625H Active Recombinant Human CCL21 Protein E.coli N/A
Human CCL21-0626H Recombinant Human CCL21 Protein, GST-Tagged Wheat Germ GST
Human CCL21-552HCL Recombinant Human CCL21 cell lysate Insect Cell N/A
Human CCL21-2653H Recombinant Human CCL21 protein, GST-tagged E.coli GST 24-134aa
Human CCL21-151H Recombinant Human CCL21 Protein, DYKDDDDK-tagged Human Cells DYKDDDDK
Human CCL21-2148H Recombinant Human CCL21 Protein, His-tagged E.coli N-His Ser24-Pro134
Human CCL21-2886H Recombinant Human CCL21 protein E.coli N/A 111
Human CCL21-19H Recombinant Human CCL21 Protein E.coli
Human CCL21-48H Recombinant Human CCL21 Protein E.coli 111 amino acid
Human CCL21-122C Active Recombinant Human CCL21 Protein (111 aa) E.coli 111
Human CCL21-62H Recombinant Human CCL21 Protein, Biotin-tagged E.coli Biotin
Human CCL21-4372H-B Recombinant Human CCL21 Protein Pre-coupled Magnetic Beads HEK293
Human CCL21-2963HF Recombinant Full Length Human CCL21 Protein, GST-tagged In Vitro Cell Free System GST 134 amino acids
Human CCL21-4372H Recombinant Human CCL21 Protein, His (Fc)-Avi-tagged HEK293 His (Fc)-Avi
Mouse CCL-328M Active Recombinant Mouse Chemokine (C-C Motif) Ligand 21A (Serine) E.coli N/A
Mouse CCL21-328M Recombinant Mouse CCL21 protein E.coli N/A 110
Rat Ccl21-7139R Recombinant Rat Ccl21 protein, His & GST-tagged E.coli His/GST Leu5~Ser132
Cynomolgus Monkey CCL21-373C Recombinant Cynomolgus CCL21 Protein, His-tagged Mammalian Cell His
Cynomolgus Monkey CCL21-424CCL Recombinant Cynomolgus CCL21 cell lysate Insect Cell N/A
Cynomolgus Monkey CCL21-121C Recombinant Cynomolgus Monkey CCL21 Protein, His (Fc)-Avi-tagged HEK293 His (Fc)-Avi
Cynomolgus Monkey CCL21-121C-B Recombinant Cynomolgus Monkey CCL21 Protein Pre-coupled Magnetic Beads HEK293
Rhesus Macaque CCL21-1197R Recombinant Rhesus CCL21 Protein Insect Cell N/A
Rhesus Macaque CCL21-685R Recombinant Rhesus monkey CCL21 Protein, His-tagged Mammalian Cell His
Rhesus Macaque CCL21-513R Recombinant Rhesus Macaque CCL21 Protein, His (Fc)-Avi-tagged HEK293 His (Fc)-Avi
Rhesus Macaque CCL21-513R-B Recombinant Rhesus Macaque CCL21 Protein Pre-coupled Magnetic Beads HEK293
  • Background
  • Quality Guarantee
  • Case Study
  • Involved Pathway
  • Protein Function
  • Interacting Protein
  • Other Resource

What is CCL21 protein?

CCL21 (C-C motif chemokine ligand 21) gene is a protein coding gene which situated on the short arm of chromosome 9 at locus 9p13. This antimicrobial gene is one of several CC cytokine genes clustered on the p-arm of chromosome 9. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. The CCL21 protein is consisted of 134 amino acids and its molecular mass is approximately 14.6 kDa.

What is the function of CCL21 protein?

Similar to other chemokines the protein encoded by this gene inhibits hemopoiesis and stimulates chemotaxis. This protein is chemotactic in vitro for thymocytes and activated T cells, but not for B cells, macrophages, or neutrophils. The cytokine encoded by CCL21 gene may also play a role in mediating homing of lymphocytes to secondary lymphoid organs. It is a high affinity functional ligand for chemokine receptor 7 that is expressed on T and B lymphocytes and a known receptor for another member of the cytokine family (small inducible cytokine A19).

CCL21-9.jpg

Fig1. Biological functions of CCL21/CCR7 axis. (Le Han, 2023)

CCL21 Related Signaling Pathway

The signaling pathway of CCL21 involves the CCR7 signaling pathway, which regulates cell migration and survival mainly through the activation of PI3K/Akt, MAPK and NF-κB. In addition, CCL21 can chemotactic T cells, B lymphocytes, mature dendritic cells (DC) and NK cells, and participate in the homing process of lymphocytes. In addition, tumor cells secrete immunosuppressive factors such as CCL21 to block the host's anti-tumor immune response, thus achieving immune escape.

CCL21 Related Diseases

It has attracted much attention because of its important role in immune cell migration and inflammation regulation. Diseases associated with CCL21 include inflammatory diseases, autoimmune diseases, and tumors, and its secretion in some aggressive tumor cells may promote tumor development.

Bioapplications of CCL21

Some relevant studies have explored its potential role in cancer treatment, including improving the effectiveness of immunotherapy by modulating the activity of immune cells in the tumor microenvironment, and there are also studies trying to exploit the biological role of CCL21 to develop new drugs or therapies targeting immunotherapy.

High Purity

High Bioactivity

CCL21-2.jpg

Fig2. Activity Data. (CCL21-2148H)

Case study 1: C Y Hong, 2014

The potential use of lymphoid chemokines to generate a dendritic cell (DC) cancer vaccine is not yet clear. The researchers investigated the effect of lymphoid chemokines on DC function and on the production of effective cytotoxic T lymphocytes (CTLs) for application of cancer vaccine using monocyte-derived mature DCs (mDCs) prestimulated with lymphoid chemokines.

mDCs exposed to a secondary lymphoid organ chemokine (SLC/CCL21) dramatically induced CTL response by increasing cytolytic activity without any significant alterations on expression of cell surface markers. mDCs prestimulated with CCL21 showed higher levels of CXCL10 (IP-10) production, but not the production of CCL22, compared with untreated mDCs. IP-10 treatment during CTL generation with DCs dramatically enhanced tumour-specific CTL response compared with untreated CTLs, and these enhanced CTL-inducing functions of CCL21-treated DCs were inhibited by anti-IP-10 treatment.

CCL21-3.jpg

Fig1. Enhanced cytotoxicity of CTLs by CCL21-treated αDC1s. The increased number of IFNγ-secreting cells in CCL21-treated αDC1s.

CCL21-4.jpg
Fig2. The dramatic enhancement of the MART-1+/CD8+ T cell population in CTLs stimulated by CCL21-treated αDC1s with or without IP-10 addition.

Case study 2: Sarah R Pickens, 2012

To determine the role of CCL21 and its receptor CCR7 in the pathogenesis of rheumatoid arthritis (RA). The role of CCL21 and/or CCR7 in angiogenesis was examined using in vitro chemotaxis, tube formation, and in vivo Matrigel plug assays. Finally, the mechanism by which CCL21 mediates angiogenesis was determined by Western blot analysis and endothelial cell chemotaxis and tube formation assays.

CCL21, but not CCL19, at concentrations present in the RA joint, induced human microvascular endothelial cell (HMVEC) migration that was mediated through CCR7 ligation. Suppression of the phosphatidylinositol 3-kinase pathway markedly reduced CCL21-induced HMVEC chemotaxis and tube formation. Neutralization of either CCL21 in RA synovial fluid or CCR7 in HMVECs significantly reduced the induction of HMVEC migration and/or tube formation by RA synovial fluid.

CCL21-5.jpg

Fig3. CCL21-induced HMVEC chemotaxis showed that CCL21 induced cell migration.

CCL21-6.jpg
Fig4. CCL21-induced HMVEC migration and tube formation are supressed by PI3K inhibition.
CCL21-7.jpg

Fig1. Schematic diagram of possible signaling pathways involved by CCL21/CCR7 axis. (Le Han, 2023)

CCL21-8.jpg

Fig2. The direct and indirect angiogenic impact of CCL21 in RA. (Katrien Van Raemdonck, 2020)

CCL21 involved in several pathways and played different roles in them. We selected most pathways CCL21 participated on our site, such as Cytokine-cytokine receptor interaction, Chemokine signaling pathway, NF-kappa B signaling pathway, which may be useful for your reference. Also, other proteins which involved in the same pathway with CCL21 were listed below. Creative BioMart supplied nearly all the proteins listed, you can search them on our site.

Pathway Name Pathway Related Protein
Cytokine-cytokine receptor interactionIL10;TNFRSF10C;TNFRSF10A;XCL2;CCL22;CCR1;TNFRSF19;TGFB3;BMP7A
Chemokine signaling pathwayGNB4;CCR5;PF4V1;CCL20;NCF1;MAPK1;PRKACG;IL-8;STAT2
NF-kappa B signaling pathwayPRKCQ;TNFRSF1A;CCL19;CFLAR;CARD11;TRAF2;TRAF6;TNFSF14;GM13304

CCL21 has several biochemical functions, for example, CCR chemokine receptor binding, CCR7 chemokine receptor binding, chemokine activity. Some of the functions are cooperated with other proteins, some of the functions could acted by CCL21 itself. We selected most functions CCL21 had, and list some proteins which have the same functions with CCL21. You can find most of the proteins on our site.

Function Related Protein
CCR chemokine receptor bindingCCL38A.5;CCL19A.2;CXCL32B.1;CCL34B.4;CCL44;CCL8;CCL19A.1;XCL1;CCL26
CCR7 chemokine receptor bindingCCL21;CCL19
chemokine activityCXCL2;CXCL9;CCL34A.4;CCL5;XCL1;CCL38.1;CCL6;CXCL18B;CCL34B.9
chemokine receptor bindingCCL19;CCL21B;Ccl21a;Ccl21c;S100A14;CCL21;CCRL2;XCL1;CCL5

CCL21 has direct interactions with proteins and molecules. Those interactions were detected by several methods such as yeast two hybrid, co-IP, pull-down and so on. We selected proteins and molecules interacted with CCL21 here. Most of them are supplied by our site. Hope this information will be useful for your research of CCL21.

LRRK1; LRRK2; a41_vaccw; CCR7; GOT2; q81ue9_bacan; UBQLN4

Gene Family

Chemokines Cytokines

Research Area

Related articles

Carubbi, F; Alunno, A; et al. Is minor salivary gland biopsy more than a diagnostic tool in primary Sjogren's syndrome? Association between clinical, histopathological, and molecular features: A retrospective study. SEMINARS IN ARTHRITIS AND RHEUMATISM 44:314-324(2014).
Chadzinska, M; Golbach, L; et al. Characterization and expression analysis of an interferon-gamma 2 induced chemokine receptor CXCR3 in common carp (Cyprinus carpio L.). DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 47:68-76(2014).
  • Reviews
  • Q&As

Customer Reviews (3)

Write a review
Reviews
11/21/2022

    CCL21 products are reliable and stable, and are widely used in scientific research, industrial production and medical fields.

    04/10/2022

      CCL21 has a long half-life and can maintain stability and reliability during the experiment.

      06/28/2021

        When using CCL21 protein for experiments, it can meet specific experimental requirements and research purposes.

        Q&As (6)

        Ask a question
        Does CCL21 have relationships with other genes or proteins? 02/18/2022

        This has a complex relationship with other genes or proteins, and can interact with other genes or proteins to participate in a variety of biochemical reactions.

        What are the applications of CCL21 in medical treatment? 02/27/2021

        It can be used as a biomarker in medical treatment to help monitor disease progression and treatment efficacy. In addition, therapeutics targeting CCL21, such as targeted therapies or modulating its expression, are also being investigated.

        What diseases can CCL21 deficiency or aberrant expression cause? 02/02/2021

        CCL21 deficiency or aberrant expression may be associated with some autoimmune diseases, cancer, and neurodegenerative diseases.

        Might abnormal CCL21 levels be indicative of disease? 12/26/2020

        Abnormal levels of CCL21 may suggest autoimmune diseases, cancer, and neurodegenerative diseases, but they may also be associated with other diseases.

        How to study the regulatory mechanism of CCL21? 10/01/2020

        Studying the regulatory mechanism of CCL21 requires a combination of various experimental methods and techniques, such as gene knockout, transcriptome analysis, and protein-protein interactions.

        How are mutations in CCL21 detected and analyzed? 03/06/2020

        Mutations in CCL21 can be detected and analyzed by methods such as whole-genome sequencing or target region sequencing to understand the impact of mutations on protein structure and function.

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