Recombinant Human CTLA4 protein, His-tagged, low endotoxin

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Recombinant Human CTLA4 protein, His-tagged, low endotoxin

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Cat.No. : CTLA4-862H
Product Overview : Recombinant Human CTLA4 (Ala37-Phe162) protein (low endotoxin) was fused to His-tag at C-terminus and expressed in human 293 cells (HEK293).
Description : CTLA-4 (Cytotoxic T-Lymphocyte Antigen 4) is also known as CD152 (Cluster of differentiation 152), is a protein receptor that downregulates the immune system. CTLA4 is a member of the immunoglobulin superfamily, which is expressed on the surface of Helper T cells and transmits an inhibitory signal to T cells. The protein contains an extracellular V domain, a transmembrane domain, and a cytoplasmic tail. Alternate splice variants, encoding different isoforms. CTLA4 is similar to the T-cell co-stimulatory protein, CD28, and both molecules bind to CD80 and CD86, also called B7-1 and B7-2 respectively, on antigen-presenting cells. CTLA4 transmits an inhibitory signal to T cells, whereas CD28 transmits a stimulatory signal. Intracellular CTLA4 is also found in regulatory T cells and may be important to their function. T cell activation through the T cell receptor and CD28 leads to increased expression of CTLA-4, an inhibitory receptor for B7 molecules. Fusion proteins of CTLA4 and antibodies (CTLA4-Ig) have been used in clinical trials for rheumatoid arthritis.
Source : HEK293
Species : Human
Tag : His
Predicted N Terminal : Ala37
Form : Lyophilized from 0.22 μm filtered solution in PBS, pH7.4, 10% trehalose.
Molecular Mass : The protein has a calculated MW of 15.4 kDa. The protein migrates as 25-30 kDa under reducing (R) condition (SDS-PAGE) due to different extent glycosylation.
Protein length : Ala37-Phe162
Endotoxin : Less than 0.01 EU per μg by the LAL method.
Purity : >95% as determined by SDS-PAGE.
Storage : For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20 to -70 centigrade for 12 months in lyophilized state;
-70 centigrade for 3 months under sterile conditions after reconstitution.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.
SDS-PAGE of CTLA4-862H :

Human CTLA-4, His Tag, low endotoxin on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA of CTLA4-862H :

Immobilized Human B7-1, Fc Tag at 2 μg/mL (100 μL/well) can bind Human CTLA-4, His Tag, low endotoxin with a linear range of 0.2-3 ng/mL (QC tested).

Bioactivity-ELISA :

Immobilized Human B7-2, Fc Tag at 2 μg/mL (100 μL/well) can bind Human CTLA-4, His Tag, low endotoxin with a linear range of 1-6 ng/mL (Routinely tested).

Gene Name : CTLA4
Official Symbol : CTLA4
Synonyms : CTLA4; cytotoxic T-lymphocyte-associated protein 4; celiac disease 3, CELIAC3; cytotoxic T-lymphocyte protein 4; CD; CD28; CD152; GSE; ICOS; CD152 isoform; celiac disease 3; cytotoxic T-lymphocyte antigen 4; cytotoxic T-lymphocyte-associated antigen 4; cytotoxic T-lymphocyte-associated serine esterase-4; cytotoxic T lymphocyte associated antigen 4 short spliced form; ligand and transmembrane spliced cytotoxic T lymphocyte associated antigen 4; GRD4; CTLA-4; IDDM12; CELIAC3
Gene ID : 1493
mRNA Refseq : NM_001037631
Protein Refseq : NP_001032720
MIM : 123890
UniProt ID : P16410

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