Recombinant Rat Ctla4 Protein, His-tagged, Alexa Fluor 555 conjugated

• Cat.No.: Ctla4-1144RAF555
• Product Overview: Alexa Fluor 555 conjugated recombinant rat Ctla4 (NP_113862.1) (Met1-Asp161) was expressed with a polyhistidine tag at the C-terminus.

Specification

• Species: Rat
• Source: HEK293
• Tag: His
• Protein Length: 137
• Form: Lyophilized
• Molecular Mass: The recombinant rat Ctla4 consists 137 amino acids and predicts a molecular mass of 15.2 kDa.
• N-terminal Sequence Analysis: Glu 36
• Endotoxin: < 1.0 EU/ μg of the protein as determined by the LAL method.
• Purity: > 95 % as determined by SDS-PAGE
• Characteristic: Disulfide-linked homodimer
  Labeled with Alexa Fluor 555 via amines
  With an excitation and emission maximum of 555/565 nm, Alexa Fluor 555 can be efficiently excited using a 543 nm He-Ne laser line and detected under standard TRITC/Cy3 filters.
• Stability: Samples are stable for up to 12 months from date of receipt at -70 centigrade.
• Storage: Store it under sterile conditions at -20 to-70 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Storage Buffer: Lyophilized from sterile PBS, pH 7.4, 5%-8% trehalose and mannitol.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 μg/μL. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.
• Conjugation: Alexa Fluor 555

Gene Information

• Gene Name: Ctla4 cytotoxic T-lymphocyte-associated protein 4 [ Rattus norvegicus ]
• Official Symbol: Ctla4
• Synonyms: CTLA4; cytotoxic T-lymphocyte-associated protein 4; cytotoxic T-lymphocyte protein 4; soluble form; CD152 antigen; transmembrane form; Cd152; CTLA-4; sCTLA4
• Gene ID: 63835
• mRNA Refseq: NM_031674
• Protein Refseq: NP_113862