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Recombinant Human CTLA4 protein, His-tagged (MALS verified)
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| Cat.No. : | CTLA4-8722H |
| Product Overview : | Recombinant Human CTLA4 protein (MALS verified)(Ala37-Phe162) was fused to His-tag at the C-terminus and expressed in human 293 cells (HEK293). |
| Description : | CTLA-4 (Cytotoxic T-Lymphocyte Antigen 4) is also known as CD152 (Cluster of differentiation 152), is a protein receptor that downregulates the immune system. CTLA4 is a member of the immunoglobulin superfamily, which is expressed on the surface of Helper T cells and transmits an inhibitory signal to T cells. The protein contains an extracellular V domain, a transmembrane domain, and a cytoplasmic tail. Alternate splice variants, encoding different isoforms. CTLA4 is similar to the T-cell co-stimulatory protein, CD28, and both molecules bind to CD80 and CD86, also called B7-1 and B7-2 respectively, on antigen-presenting cells. CTLA4 transmits an inhibitory signal to T cells, whereas CD28 transmits a stimulatory signal. Intracellular CTLA4 is also found in regulatory T cells and may be important to their function. T cell activation through the T cell receptor and CD28 leads to increased expression of CTLA-4, an inhibitory receptor for B7 molecules. Fusion proteins of CTLA4 and antibodies (CTLA4-Ig) have been used in clinical trials for rheumatoid arthritis. |
| Source : | HEK293 |
| Species : | Human |
| Tag : | His |
| Predicted N Terminal : | Ala37 |
| Form : | Lyophilized from 0.22 μm filtered solution in PBS, pH7.4, 10% trehalose. |
| Molecular Mass : | The protein has a calculated MW of 15.4 kDa. As a result of glycosylation, the protein migrates as 23-27 kDa under reducing (R) condition, and 38-50 kDa under non-reducing (NR) condition (SDS-PAGE). |
| Protein length : | Ala37-Phe162 |
| Endotoxin : | Less than 1.0 EU per μg by the LAL method. |
| Purity : | >90% as determined by SDS-PAGE. >90% as determined by SEC-MALS. |
| Storage : | For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. |
| Reconstitution : | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4°C before opening to recover the entire contents. |
| SDS-PAGE of CTLA4-8722H : |
Human CTLA-4, His Tag (MALS verified) on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%. |
| SEC-MALS of CTLA4-8722H : |
The purity of Human CTLA-4, His Tag (MALS verified) was more than 90% in HP-SEC, and around 40-55 kDa verified by SEC-MALS. |
| Bioactivity-ELISA of CTLA4-8722H : |
Immobilized Human B7-1, Fc Tag (HPLC-verified) at 2 μg/mL (100 μL/well) can bind Human CTLA-4, His Tag (MALS verified) with a linear range of 0.2-2 ng/mL (QC tested). 
Immobilized Human B7-2, Fc Tag at 2 μg/mL (100 μL/well) can bind Human CTLA-4, His Tag (MALS verified) with a linear range of 0.2-2 ng/mL (Routinely tested). |
| Bioactivity-SPR of CTLA4-8722H : |
Yervoy (Ipilimumab) captured on CM5 chip via Anti-human IgG Fc antibodies surface can bind Human CTLA-4, His Tag (MALS verified) with an affinity constant of 55.2 pM as determined in a SPR assay (Biacore 8K) (Routinely tested). |
| Bioactivity-BLI of CTLA4-8722H : |
Loaded Human B7-1, Fc Tag (HPLC-verified) on Protein A Biosensor, can bind Human CTLA-4, His Tag (MALS verified) with an affinity constant of 1.44 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested). 
Loaded Human B7-2, Fc Tag on Protein A Biosensor, can bind Human CTLA-4, His Tag (MALS verified) with an affinity constant of 3.54 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested). |
| Gene Name : | CTLA4 |
| Official Symbol : | CTLA4 |
| Synonyms : | CD; GSE; GRD4; ALPS5; CD152; CTLA-4; IDDM12; CELIAC3 |
| Gene ID : | 1493 |
| mRNA Refseq : | NM_001037631.3 |
| Protein Refseq : | NP_001032720.1 |
| MIM : | 123890 |
| UniProt ID : | P16410 |
◆ Recombinant Protein
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| CTLA4-3358M | Recombinant Mouse CTLA4 Protein, Fc-tagged | +Inquiry |
| CTLA4-257H | Recombinant Human CTLA4 Protein, His-Avi-tagged, Biotinylated | +Inquiry |
| CTLA4-500H | Recombinant Human CTLA4 Protein (Met1-Asp161) | +Inquiry |
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◆ Lysates
| CTLA4-1047CCL | Recombinant Cynomolgus CTLA4 cell lysate | +Inquiry |
| CTLA4-2526HCL | Recombinant Human CTLA4 cell lysate | +Inquiry |
| CTLA4-2179MCL | Recombinant Mouse CTLA4 cell lysate | +Inquiry |
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◆ Assay kits
| Kit-1712 | CTLA4:B7-1[Biotinylated] Inhibitor Screening Assay Kit | +Inquiry |
| Kit-1713 | CTLA4[Biotinylated]:B7-2 Inhibitor Screening Assay Kit | +Inquiry |
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