Recombinant Human CTLA4 protein, His-tagged (MALS verified) CTLA4-8722H

Recombinant Human CTLA4 protein, His-tagged (MALS verified)

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Recombinant Human CTLA4 protein, His-tagged (MALS verified)

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Cat.No. : CTLA4-8722H
Product Overview : Recombinant Human CTLA4 protein (MALS verified)(Ala37-Phe162) was fused to His-tag at the C-terminus and expressed in human 293 cells (HEK293).
Description : CTLA-4 (Cytotoxic T-Lymphocyte Antigen 4) is also known as CD152 (Cluster of differentiation 152), is a protein receptor that downregulates the immune system. CTLA4 is a member of the immunoglobulin superfamily, which is expressed on the surface of Helper T cells and transmits an inhibitory signal to T cells. The protein contains an extracellular V domain, a transmembrane domain, and a cytoplasmic tail. Alternate splice variants, encoding different isoforms. CTLA4 is similar to the T-cell co-stimulatory protein, CD28, and both molecules bind to CD80 and CD86, also called B7-1 and B7-2 respectively, on antigen-presenting cells. CTLA4 transmits an inhibitory signal to T cells, whereas CD28 transmits a stimulatory signal. Intracellular CTLA4 is also found in regulatory T cells and may be important to their function. T cell activation through the T cell receptor and CD28 leads to increased expression of CTLA-4, an inhibitory receptor for B7 molecules. Fusion proteins of CTLA4 and antibodies (CTLA4-Ig) have been used in clinical trials for rheumatoid arthritis.
Source : HEK293
Species : Human
Tag : His
Predicted N Terminal : Ala37
Form : Lyophilized from 0.22 μm filtered solution in PBS, pH7.4, 10% trehalose.
Molecular Mass : The protein has a calculated MW of 15.4 kDa. As a result of glycosylation, the protein migrates as 23-27 kDa under reducing (R) condition, and 38-50 kDa under non-reducing (NR) condition (SDS-PAGE).
Protein length : Ala37-Phe162
Endotoxin : Less than 1.0 EU per μg by the LAL method.
Purity : >90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Storage : For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4°C before opening to recover the entire contents.
SDS-PAGE of CTLA4-8722H :

Human CTLA-4, His Tag (MALS verified) on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

SEC-MALS of CTLA4-8722H :

The purity of Human CTLA-4, His Tag (MALS verified) was more than 90% in HP-SEC, and around 40-55 kDa verified by SEC-MALS.

Bioactivity-ELISA of CTLA4-8722H :

Immobilized Human B7-1, Fc Tag (HPLC-verified) at 2 μg/mL (100 μL/well) can bind Human CTLA-4, His Tag (MALS verified) with a linear range of 0.2-2 ng/mL (QC tested).

Immobilized Human B7-2, Fc Tag at 2 μg/mL (100 μL/well) can bind Human CTLA-4, His Tag (MALS verified) with a linear range of 0.2-2 ng/mL (Routinely tested).

Bioactivity-SPR of CTLA4-8722H :

Yervoy (Ipilimumab) captured on CM5 chip via Anti-human IgG Fc antibodies surface can bind Human CTLA-4, His Tag (MALS verified) with an affinity constant of 55.2 pM as determined in a SPR assay (Biacore 8K) (Routinely tested).

Bioactivity-BLI of CTLA4-8722H :

Loaded Human B7-1, Fc Tag (HPLC-verified) on Protein A Biosensor, can bind Human CTLA-4, His Tag (MALS verified) with an affinity constant of 1.44 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Loaded Human B7-2, Fc Tag on Protein A Biosensor, can bind Human CTLA-4, His Tag (MALS verified) with an affinity constant of 3.54 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Gene Name : CTLA4
Official Symbol : CTLA4
Synonyms : CD; GSE; GRD4; ALPS5; CD152; CTLA-4; IDDM12; CELIAC3
Gene ID : 1493
mRNA Refseq : NM_001037631.3
Protein Refseq : NP_001032720.1
MIM : 123890
UniProt ID : P16410

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