Active Recombinant Human CTLA4 Homodimer Protein, His tagged

• Cat.No.: CTLA4-35H
• Product Overview: CTLA-4 protein dimer contains a CTLA-4 extracellular domain (Uniprot# P16410) fused with a proprietary dimer motif followed by a His tag at the C-terminus was expressed in HEK293T.

Specification

• Species: Human
• Source: HEK293T
• Tag: His
• Protein Length: 36-161 aa
• Description: Cytotoxic T-lymphocyte associated protein 4 (CTLA-4), also known as CD152 (cluster of differentiation 152), CD152 antigen, CTLA4, ALPS5, CD, CELIAC3, GRD4, GSE, and IDDM12 is a member of the immunoglobulin superfamily. CTLA-4 contains an extracellular immunoglobulin-like (Ig-like) domain (an Ig-V-like and an Ig-C-like domain), a transmembrane domain, and a cytoplasmic tail. As an immune checkpoint CTLA-4 binds both CD80 (Cluster of differentiation 80) and CD86 (Cluster of differentiation 86) to transmit an inhibitory signal to T cells, competing with CD28 (Cluster of differentiation 28) which transmits a stimulatory signal. It is often overexpressed in malignancies caused by immunosurveillance, making the inhibition of immune checkpoint proteins like CTLA-4 an emerging strategy in cancer therapy. CTLA-4 gene variants have been associated with Type 1 diabetes, Graves' disease, Hashimoto's thyroiditis, celiac disease, and other autoimmune diseases.
• Molecular Mass: 50 kDa
• Purity: Greater than 90% dimer form as determined by SDS-PAGE under non-reducing condition
• Application: Verified Applications: ELISA, Western blot for CTLA-4-specific antibody and CD80 ligand protein binding assays. BLI protein binding assays for CTLA-4 with CD80. Suggested Applications: SPR & BLI for CTLA-4-specific antibody or CD80 ligand protein binding assays. Western blot with CTLA-4-specific antibody. ELISA for CTLA-4-specific antibody or CD80 ligand protein binding assays. Animal immunization, RUO.
• Storage: Store at -80 centigrade.
• Storage Buffer: 0.22μm filtered PBS, pH 7.4
• Shipping: Frozen Dry Ice

Gene Information

• Gene Name: CTLA4 cytotoxic T-lymphocyte-associated protein 4 [ Homo sapiens (human) ]
• Official Symbol: CTLA4
• Synonyms: CTLA4; cytotoxic T-lymphocyte-associated protein 4; celiac disease 3, CELIAC3; cytotoxic T-lymphocyte protein 4; CD; CD28; CD152; GSE; ICOS; CD152 isoform; celiac disease 3; cytotoxic T-lymphocyte antigen 4; cytotoxic T-lymphocyte-associated antigen 4; cytotoxic T-lymphocyte-associated serine esterase-4; cytotoxic T lymphocyte associated antigen 4 short spliced form; ligand and transmembrane spliced cytotoxic T lymphocyte associated antigen 4; GRD4; CTLA-4; IDDM12; CELIAC3
• Gene ID: 1493
• mRNA Refseq: NM_001037631
• Protein Refseq: NP_001032720
• MIM: 123890
• UniProt ID: P16410