Recombinant Human CXCR3 Full Length Transmembrane protein, Flag-tagged(Nanodisc)

• Cat.No.: CXCR3-758H
• Product Overview: Recombinant Human CXCR3 Full Length Transmembrane protein(P49682), fused with Flag tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Human
• Tag: Flag
• Form: Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions.
• Bio-activity: Elisa plates were pre-coated with Flag Tag CXCR3-Nanodisc (0.2μg/per well). Serial diluted anti-CXCR3 monoclonal antibody (DME100208) solutions were added, washed, and incubated with secondary antibody before Elisa reading. From above data, the EC50 for anti-CXCR3 monoclonal antibody binding with CXCR3-Nanodisc is 15.79ng/ml.
• Molecular Mass: The human full length CXCR3 protein has a MW of 40.6 kDa
• Storage: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: CXCR3 chemokine (C-X-C motif) receptor 3 [ Homo sapiens ]
• Official Symbol: CXCR3
• Synonyms: CXCR3; chemokine (C-X-C motif) receptor 3; G protein coupled receptor 9 , GPR9; C-X-C chemokine receptor type 3; CD183; CKR L2; CMKAR3; IP10 R; MigR; CXC-R3; CXCR-3; Mig receptor; IP10 receptor; IP-10 receptor; G protein-coupled receptor 9; chemokine (C-X-C) receptor 3; interferon-inducible protein 10 receptor; GPR9; CD182; Mig-R; CKR-L2; IP10-R
• Gene ID: 2833
• mRNA Refseq: NM_001142797
• Protein Refseq: NP_001136269
• MIM: 300574
• UniProt ID: P49682

Reviews

12/11/2019

The continuous innovation and improvements made by the manufacturer indicated a promising future for the product.

07/30/2019

Inclusion of the product consistently led to a significant upregulation of target protein expression levels.

12/19/2016

We were excited about the product's role in driving future discoveries in our field of study.

Q&As

What is the primary role of chemokines and their receptors, particularly CXCR3, in immune cell processes?

The primary role of chemokines and their receptors, including CXCR3, is in the recruitment, activation, and differentiation of immune cells.

How many variant isoforms of CXCR3 exist, and what are their names?

There are three variant isoforms of CXCR3: CXCR3A, CXCR3B, and CXCR3alt.

Which ligands are associated with the chemokine receptor CXCR3, and what are their roles during interferon-induced inflammatory responses?

The ligands associated with the chemokine receptor CXCR3 are CXCL9, CXCL10, and CXCL11, and they play key roles during interferon-induced inflammatory responses.

What aspect of the inflammatory process in human skin involving CXCR3 chemokines and isoforms requires further investigation, according to the information provided?

The relative contributions of different CXCR3 chemokines and the three variant isoforms to the inflammatory process in human skin require further investigation.

What is the focus of the review regarding CXCR3, its ligands, and their role in skin during inflammation and carcinogenesis?

The review focuses on the biology of CXCR3 and its associated ligands, with particular emphasis on their roles in the skin during inflammation and carcinogenesis.

In the context of skin cancers, what dual role can the CXCR3 receptor play, and how does its expression on different cell types contribute to cancer outcomes?

In skin cancers, the CXCR3 receptor can play a dual role. Its expression on tumor cells can lead to cancer metastasis, while expression on immune cells can promote anti-tumor immune responses.

What drives the expression of CXCL9, CXCL10, and CXCL11, and what is their function in recruiting CXCR3+ T cells during skin inflammation?

The expression of CXCL9, CXCL10, and CXCL11 is driven by inflammation of the skin resulting from infections or autoimmune disease, leading to the recruitment of effector, CXCR3+ T cells from the circulation.