Active Recombinant Human / Cynomolgus / Rhesus Monkey CD28 Protein, Fc & Avi-tagged, Biotinylated

• Cat.No.: CD28-338H
• Product Overview: Recombinant Human / Cynomolgus / Rhesus Monkey CD28 Protein, Fc & Avi-tagged, Biotinylated is expressed from human 293 cells (HEK293). It contains AA Asn 19 - Pro 152 (Accession # AAH93698). Predicted N-terminus: Asn 19. This protein carries a human IgG1 Fc tag at the C-terminus, followed by a Avi tag. The protein has a calculated MW of 43.6 kDa. As a result of glycosylation, the protein migrates as 55-66 kDa under reducing (R) condition, and 110-130 kDa under non-reducing (NR) condition (SDS-PAGE).

Specification

• Source: HEK293
• Species: Human / Cynomolgus / Rhesus Monkey
• Tag: Fc & Avi
• Form: Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5. Normally trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Human B7-2, Fc Tag at 5 μg/mL (100 μL/well) can bind Recombinant Human / Cynomolgus / Rhesus Monkey CD28 Protein, Fc & Avi-tagged, Biotinylated with a linear range of 0.15-5 μg/mL (QC tested).
  Immobilized Anti-CD28 MAb, Mouse IgG1 at 5 μg/mL (100 μL/well) can bind Recombinant Human / Cynomolgus / Rhesus Monkey CD28 Protein, Fc & Avi-tagged, Biotinylated with a linear range of 2.4-39 ng/mL (Routinely tested).
  Immobilized Human B7-1, Fc Tag at 5 μg/mL (100 μL/well) can bind Recombinant Human / Cynomolgus / Rhesus Monkey CD28 Protein, Fc & Avi-tagged, Biotinylated with a linear range of 0.313-5 μg/mL (Routinely tested).
• Molecular Mass: 43.6 kDa
• Protein length: Asn 19 - Pro 152
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Notes: Please avoid repeated freeze-thaw cycles.
• Stability: No activity loss is observed after storage at:
  4-8 centigrade for 12 months in lyophilized state;
  -70 centigrade for 3 months under sterile conditions after reconstitution.
• Storage: For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.

Gene Information

• Gene Name: CD28 CD28 molecule [ Homo sapiens ]
• Official Symbol: CD28
• Synonyms: CD28; CD28 molecule; CD28 antigen (Tp44); CD28 antigen; Tp44; MGC138290
• Gene ID: 940
• mRNA Refseq: NM_001243077
• Protein Refseq: NP_001230006
• MIM: 186760
• UniProt ID: P10747