Active Recombinant Human/Cynomolgus/Rhesus macaque CD28 protein, mFc-tagged, low endotoxin

• Cat.No.: CD28-336H
• Product Overview: Recombinant Human/Cynomolgus/Rhesus macaque CD28 protein (Asn19-Pro152)(low endotoxin), fused to Mouse IgG2a Fc tag at C-terminus, was expressed in human 293 cells (HEK293).In the region Asn19-Pro152, the AA sequence of Human, Cynomolgus and Rhesus macaque CD28 are homologus.

Specification

• Source: HEK293
• Species: Human
• Tag: mFc
• Form: Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5. Normally trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Human / Cynomolgus / Rhesus macaque CD28, Mouse IgG2a Fc Tag at 5 μg/mL (100 μL/well) can bind Human B7-2, Fc Tag with a linear range of 0.1-5 μg/mL.
• Molecular Mass: The protein has a calculated MW of 42.0 kDa. The protein migrates as 50-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Protein length: Asn 19 - Pro 152
• Endotoxin: Less than 0.1 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
  >90% as determined by SEC-MALS.
• Storage: For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
  Please avoid repeated freeze-thaw cycles.
  This product is stable after storage at:
  -20 centigrade to -70 centigrade for 12 months in lyophilized state;
  -70 centigrade for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: CD28 CD28 molecule [ Homo sapiens ]
• Official Symbol: CD28
• Synonyms: CD28; CD28 molecule; CD28 antigen (Tp44); T-cell-specific surface glycoprotein CD28; T cell specific surface glycoprotein; CD28 antigen; Tp44; MGC138290
• Gene ID: 940
• mRNA Refseq: NM_001243077
• Protein Refseq: NP_001230006
• MIM: 186760
• UniProt ID: P10747

Reviews

08/24/2021

Its unique characteristics contribute to obtaining clear and detailed structural information, enabling researchers to unravel the intricate complexities of protein interactions and conformational changes.

08/24/2021

Researchers can confidently rely on its capabilities to generate precise and significant results, enhancing the understanding of protein function and dynamics.

08/06/2021

the CD28 protein's outstanding performance in ELISA and its utility in protein electron microscopy structure analysis make it a highly recommended tool for researchers.

Q&As

Can CD28 protein be used for personalized medicine?

CD28 protein expression and function may be relevant for personalized treatment strategies in autoimmune diseases and cancer.

What are the potential clinical applications of targeting CD28 protein?

Targeting CD28 protein may have therapeutic potential in treating autoimmune diseases, cancer immunotherapy, and organ transplantation.

How does CD28 protein impact regulatory T cells (Tregs)?

CD28 signaling is important for the function and maintenance of regulatory T cells, which play a role in immune tolerance and suppression of autoimmunity.

How does CD28 protein impact cancer immunotherapy?

CD28 protein is involved in enhancing the anti-tumor immune response, making it a potential target for cancer immunotherapy.

Are there any approved therapies targeting CD28 protein?

Currently, there are no FDA-approved therapies specifically targeting CD28 protein, but research is ongoing in this area.