Recombinant Mouse CD28 cell lysate

Cat.No. : CD28-2002MCL
Product Overview : Mouse CD28 derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls
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Source : Human cells
Species : Mouse
Preparation method : Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Lysis buffer : Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Quality control Testing : 12.5% SDS-PAGE Stained with Coomassie Blue
Recommended Usage : 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Stability : Samples are stable for up to twelve months from date of receipt at -80°C
Storage Buffer : 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Storage Instruction : Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
Gene Name : Cd28 CD28 antigen [ Mus musculus ]
Official Symbol : CD28
Synonyms : CD28; CD28 antigen; T-cell-specific surface glycoprotein CD28;
Gene ID : 12487
mRNA Refseq : NM_007642
Protein Refseq : NP_031668
Pathway : Adaptive Immune System, organism-specific biosystem; Allograft rejection, organism-specific biosystem; Allograft rejection, conserved biosystem; Autoimmune thyroid disease, organism-specific biosystem; Autoimmune thyroid disease, conserved biosystem; CD28 co-stimulation, organism-specific biosystem; CD28 dependent PI3K/Akt signaling, organism-specific biosystem;
Function : SH3/SH2 adaptor activity; protease binding; protein homodimerization activity;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.


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Q&As (5)

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Can CD28 protein be used for personalized medicine? 01/13/2023

CD28 protein expression and function may be relevant for personalized treatment strategies in autoimmune diseases and cancer.

What are the potential clinical applications of targeting CD28 protein? 05/18/2021

Targeting CD28 protein may have therapeutic potential in treating autoimmune diseases, cancer immunotherapy, and organ transplantation.

How does CD28 protein impact regulatory T cells (Tregs)? 06/21/2019

CD28 signaling is important for the function and maintenance of regulatory T cells, which play a role in immune tolerance and suppression of autoimmunity.

How does CD28 protein impact cancer immunotherapy? 11/26/2018

CD28 protein is involved in enhancing the anti-tumor immune response, making it a potential target for cancer immunotherapy.

Are there any approved therapies targeting CD28 protein? 05/27/2017

Currently, there are no FDA-approved therapies specifically targeting CD28 protein, but research is ongoing in this area.

Customer Reviews (3)

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    Its unique characteristics contribute to obtaining clear and detailed structural information, enabling researchers to unravel the intricate complexities of protein interactions and conformational changes.


      Researchers can confidently rely on its capabilities to generate precise and significant results, enhancing the understanding of protein function and dynamics.


        the CD28 protein's outstanding performance in ELISA and its utility in protein electron microscopy structure analysis make it a highly recommended tool for researchers.

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