Optional Service: Optional requirements on this protein
Product Overview :
Alexa Fluor 555 conjugated recombinant human CD28 (P10747-1) (Met 1-Pro 152) was expressed with six amino acids (LEVLFQ) at the C-terminus.
Molecular Mass :
The recombinant human CD28 consists of 141 amino acids and predicts a molecular mass of 15.9 kDa. It migrates as an approximately 23 kDa band in SDS-PAGE under reducing conditions.
Protein length :
N-terminal Sequence Analysis :
< 1.0 EU/ μg of the protein as determined by the LAL method.
> 90 % as determined by SDS-PAGE
Disulfide-linked homodimer Labeled with Alexa Fluor 555 via amines With an excitation and emission maximum of 555/565 nm, Alexa Fluor 555 can be efficiently excited using a 543 nm He-Ne laser line and detected under standard TRITC/Cy3 filters.
Samples are stable for up to 12 months from date of receipt at -70 centigrade.
Store it under sterile conditions at -20 to-70 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Storage Buffer :
Lyophilized from sterile PBS, pH 7.4, 5%-8% trehalose and mannitol.
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 μg/μL. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.