Recombinant Human DNMT3B Protein, GST-tagged

• Cat.No.: DNMT3B-2792H
• Product Overview: Human DNMT3B full-length ORF (NP_787045.1, 1 a.a. - 770 a.a.) recombinant protein with GST-tag at N-terminal.

Specification

• Description: CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a DNA methyltransferase which is thought to function in de novo methylation, rather than maintenance methylation. The protein localizes primarily to the nucleus and its expression is developmentally regulated. Mutations in this gene cause the immunodeficiency-centromeric instability-facial anomalies (ICF) syndrome. Eight alternatively spliced transcript variants have been described. The full length sequences of variants 4 and 5 have not been determined. [provided by RefSeq, May 2011]
• Source: Wheat Germ
• Species: Human
• Tag: GST
• Molecular Mass: 112.6 kDa
• AA Sequence: MKGDTRHLNGEEDAGGREDSILVNG ACSDQSSDSPPILEAIRTPEIRGRR SSSRLSKREVSSLLSYTQDLTGDGD GEDGDGSDTPVMPKLFRETRTRSES PAVRTRNNNSVSSRERHRPSPRSTR GRQGRNHVDESPVEFPATRSLRRRA TASAGTPWPSPPSSYLTIDLTDDTE DTHGTPQSSSTPYARLAQDSQQGGM ESPQVEADRGDGDSSEYQDGKEFGI GDLVWGKIKGFSWWPAMVVSWKATS KRQAMSGMRWVQWFGDGKFSEVSAD KLVALGLFSQHFNLATFNKLVSYRK AMYHALEKARVRAGKTFPSSPGDSL EDQLKPMLEWAHGGFKPTGIEGLKP NNTQPENKTRRRTADDSATSDYCPA PKHLKTNCYNNGKDRGDEDQSREQM ASDVANNKSSLEDGCLSCGRKNPVS FHPLFEGGLCQTCRDRFLELFYMYD DDGYQSYCTVCCEGRELLLCSNTSC CRCFCVECLEVLVGTGTAAEAKLQE PWSCYMCLPQRCHGVLRRRKDWNVR LQAFFTSDTGLEYEAPKLYPAIPAA RRRPIRVLSLFDGIATGYLVLKELG IKVGKYVASEVCEESIAVGTVKHEG NIKYVNDVRNITKKNIEEWGPFDLV IGGSPCNDLSNVNPARKGLYEGTGR LFFEFYHLLNYSRPKEGDDRPFFWM FENVVAMKVGDKRDISRFLECNPVM IDAIKVSAAHRARYFWGNLPGMNRI FGFPVHYTDVSNMGRGARQKLLGRS WSVPVIRHLFAPLKDYFACE
• Applications: Enzyme-linked Immunoabsorbent Assay
  Western Blot (Recombinant protein)
  Antibody Production
  Protein Array
• Notes: Best use within three months from the date of receipt of this protein.
• Storage: Store at -80 centigrade. Aliquot to avoid repeated freezing and thawing.
• Storage Buffer: 50 mM Tris-HCI, 10 mM reduced Glutathione, pH=8.0 in the elution buffer.

Gene Information

• Gene Name: DNMT3B DNA (cytosine-5-)-methyltransferase 3 beta [ Homo sapiens ]
• Official Symbol: DNMT3B
• Synonyms: DNMT3B; DNA (cytosine-5-)-methyltransferase 3 beta; DNA (cytosine-5)-methyltransferase 3B; DNA MTase HsaIIIB; DNA methyltransferase HsaIIIB; ICF; ICF1; M.HsaIIIB
• Gene ID: 1789
• mRNA Refseq: NM_001207055
• Protein Refseq: NP_001193984
• MIM: 602900
• UniProt ID: Q9UBC3