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Recombinant Human EP300 Protein, N-Flag-tagged

Cat.No. : EP300-02H
Product Overview : The wild-type p300 protein consists of full-length (2414 amino acid residues) p300 (accession number NM_001429) and contains an N-FLAG-Tag. The p300 protein was expressed in a baculovirus system and purified by an affinity column in combination with FPLC chromatography. The purified recombinant protein is greater than 95% homogeneous and contains no detectable protease, DNase and RNase activity.
  • Specification
  • Gene Information
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Description : This gene encodes the adenovirus E1A-associated cellular p300 transcriptional co-activator protein. It functions as histone acetyltransferase that regulates transcription via chromatin remodeling and is important in the processes of cell proliferation and differentiation. It mediates cAMP-gene regulation by binding specifically to phosphorylated CREB protein. This gene has also been identified as a co-activator of HIF1A (hypoxia-inducible factor 1 alpha), and thus plays a role in the stimulation of hypoxia-induced genes such as VEGF. Defects in this gene are a cause of Rubinstein-Taybi syndrome and may also play a role in epithelial cancer.
Source : Baculovirus
Species : Human
Tag : N-Flag
Applications : Recombinant p300 is suitable for protein-protein interaction, in vitro transcription, in vitro acetylation and cell growth assays. 1-5 ng is sufficient for gelshift assays in a 20 μL volume, 50-100 ng is sufficient for reconstituted transcription assays and 100-200 ng is sufficient for protein-protein interaction or acetylation assays. The molecular weight of the protein is 265 kDa. NOTE: The presence of Poly [d(I-C)] in buffers may affect protein functionality and should be avoided.
Notes : This product is guaranteed for 6 months from date of receipt.
Stability : This product is guaranteed for 6 months from date of receipt.
Storage : Recombinant proteins in solution are temperature sensitive and must be stored at -80 centigrade to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Concentration : 0.4 μg/μL
Storage Buffer : 4 μg of Recombinant p300 protein in Dilution Buffer AM1 (20 mM TrisCl (pH 8), 20% glycerol, 100 mM KCl, 1 mM DTT and 0.2 mM EDTA).
Gene Name : EP300 E1A binding protein p300 [ Homo sapiens (human) ]
Official Symbol : EP300
Synonyms : EP300; E1A binding protein p300; p300; KAT3B; MKHK2; RSTS2; histone acetyltransferase p300; E1A-associated protein p300; E1A-binding protein, 300kD; histone butyryltransferase p300; histone crotonyltransferase p300; p300 HAT; protein 2-hydroxyisobutyryltransferase p300; protein lactyltransferas p300; protein propionyltransferase p300; EC 2.3.1.48
Gene ID : 2033
mRNA Refseq : NM_001429
Protein Refseq : NP_001420
MIM : 602700
UniProt ID : Q7Z6C1

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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