Description : |
This gene encodes the adenovirus E1A-associated cellular p300 transcriptional co-activator protein. It functions as histone acetyltransferase that regulates transcription via chromatin remodeling and is important in the processes of cell proliferation and differentiation. It mediates cAMP-gene regulation by binding specifically to phosphorylated CREB protein. This gene has also been identified as a co-activator of HIF1A (hypoxia-inducible factor 1 alpha), and thus plays a role in the stimulation of hypoxia-induced genes such as VEGF. Defects in this gene are a cause of Rubinstein-Taybi syndrome and may also play a role in epithelial cancer. |
Source : |
Baculovirus |
Species : |
Human |
Tag : |
N-Flag |
Molecular Mass : |
98.7 kDa |
Protein Length : |
965-1810 |
Applications : |
Recombinant p300 protein, catalytic domain is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling. |
Usage : |
0.5 μg Histone H4 was incubated with 0 μg (-), 0.5 μg (+) p300 protein, catalytic domain in 20 μL reaction system containing 50 mM Tris-HCl pH 8.0, 0.1 mM EDTA, 50 ng/μL BSA, 1 mM TCEP and 20 μM Acetyl-CoA for 2 hours at room temperature. Half of each reaction was run on a 13% SDS-PAGE gel, and products were detected by Western blot. H4ac (pan-acetyl) antibody was used to recognize acetylated histone H4. |
Notes : |
This product is for research use only and is not for use in diagnostic procedures. |
Stability : |
This product is guaranteed for 6 months from date of arrival. |
Storage : |
Recombinant proteins in solution are temperature sensitive and must be stored at -80 centigrade to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
Concentration : |
0.23 μg/μL |
Storage Buffer : |
Recombinant p300 protein, catalytic domain is supplied in 25 mM HEPES-NaOH pH 7.5, 300 mM NaCl, 10% glycerol, 0.04% Triton X-100 and 0.5 mM TCEP. |