Recombinant Human Ephrin-A1

• Cat.No.: EFNA1-150H
• Product Overview: Recombinant Human Ephrin A1 protein expressed inE.coliencodes full length amino acids and has a molecular mass of 47 kDa.

Specification

• Species: Human
• Source: E.coli
• Tag: Non
• Description: Ephrins are a family of molecules which bind to tyrosine kinase receptor Eph. The Eph receptor and their membrane-attached ligands, ephrins, show diverse expression patterns during development. The crucial roles of Eph and ephrins during development suggest involvement of these genes in congenital disorders affecting the nervous system and other tissues. Eph receptors and ephrins may be involved in carcinogenesis.
• Formulation: 5µg/50µl Recombinant Human Ephrin A1 protein at 100µg/ml in 50mM Tris-Acetate, pH7.5, 1mM EDTA and 20% Glycerol.
• Application and suggested dilutions: •ELISA•Inhibition Assays •Western Blotting
• Characterization: On SDS-PAGE commassie blue stained gel, the purified recombinant protein shows a band at 47 kDa (Including GST).
• Storage & stability: Store vial at -20°Cto -80°C. When stored at the recommended temperature, this protein is stable for 12 months.
• Full Length: Full L.
• Publications:
  The Small Molecule Ephrin Receptor Inhibitor, GLPG1790, Reduces Renewal Capabilities of Cancer Stem Cells, Showing Anti-Tumour Efficacy on Preclinical Glioblastoma Models (2019)

Gene Information

• Gene Name: EFNA1 ephrin-A1 [ Homo sapiens ]
• Synonyms: EFNA1; B61; ECKLG; EFL1; EPLG1; LERK-1; TNFAIP4; Ephrin-A1; OTTHUMP00000033242; OTTHUMP00000033271; EPH-related receptor tyrosine kinase ligand 1; Immediate early response protein B61; Tumor necrosis factor, alpha-induced protein 4; Ligand of eph-related kinase 1
• Gene ID: 1942
• mRNA Refseq: NM_004428
• Protein Refseq: NP_004419
• MIM: 191164
• UniProt ID: P20827
• Chromosome Location: 1q21-q22
• Pathway: Axon guidance
• Function: Ephrin receptor binding

Citation

The Small Molecule Ephrin Receptor Inhibitor, GLPG1790, Reduces Renewal Capabilities of Cancer Stem Cells, Showing Anti-Tumour Efficacy on Preclinical Glioblastoma Models

Journal: Cancers    PubMed ID: 30871240    Data: 2019/3/13

Authors: Giovanni Luca Gravina, Andrea Mancini, Claudio Festuccia

Article Snippet:Cell-based enzyme-linked immunosorbent assays (ELISAs) for total and phosphorylated isoforms in Ser 897 and Tyr 588 EphA2 as well as total and p-Tyrosine779 EphA3 and total and p-Tyrosine987 EphB4 were used for detecting and quantifying target proteins in cultured cells following the “In-Cell ELISA protocol” on the Abcam website ( http://www.abcam.com/protocols/in-cell-elisa-ice ).Cell-based enzyme-linked immunosorbent assays (ELISAs) for total and phosphorylated isoforms in Ser 897 and Tyr 588 EphA2 as well as total and p-Tyrosine779 EphA3 and total and p-Tyrosine987 EphB4 were used for detecting and quantifying target proteins in cultured cells following the “In-Cell ELISA protocol” on the Abcam website ( http://www.abcam.com/protocols/in-cell-elisa-ice ).. Recombinant Human Ephrin-A1 (EFNA1-150H) and Recombinant Human Ephrin-B2 (EFNB2-480H) were purchased from Creative BioMart (Shirley, NY, USA).