Recombinant Human FGF21, His-tagged
| Cat.No. : | FGF21-104H |
| Product Overview : | Recombinant Human Fibroblast Growth Factor-21 produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 181 amino acids and an N-terminal Methionin (bold), having a molecular weight of 19.7 kDa (calculated). The amino acid sequence of the recombinant human FGF21 is 100% homologous to the amino acid sequence of the human FGF21 without signal sequence and contains 14 a. His-tag at N-terminal. |
- Specification
- Gene Information
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| Cat. No. : | FGF21-104H |
| Description : | FGF-21 is a unique member of FGF super family that is reported to be primarily produced by the liver. FGF-21 has recently been shown to have beneficial effects on a variety of metabolic parameters, including glucose and lipid metabolism and insulin sensitivity in animal models and is thought to exert potent antidiabetic and lipid-lowering agent of obesity and type 2 diabetes mellitus. FGF-21 activity depends on membrane protein beta Klotho that physically complexes with various FGF receptors, thus conferring them the ability to bind FGF-21 and activate downstream signaling pathways. |
| Source : | Escherichia Coli. |
| Amino Acid Sequence : | MGSSHHHHHH SSGLVPRGSH MHPIPDSSPL LQFGGQVRQR YLYTDDAQQT EAHLEIREDG TVGGAADQSP ESLLQLKALK PGVIQILGVK TSRFLCQRPD GALYGSLHFD PEACSFRELL LEDGYNVYQS EAHGLPLHLP GNKSPHRDPA PRGPARFLPL PGLPPAPPEP PGILAPQPPD VGSSDPLSMV GPSQGRSPSY AS |
| Form : | Liquid. In 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol |
| Molecular Weight : | 21.6 kDa (202aa), confirmed by MALDI-TOF (molecular weight on SDS-PAGE will appear higher) |
| Purity : | > 90% by SDS - PAGE |
| Concentration : | 1 mg/ml (determined by Bradford assay) |
| Endotoxin Level : | < 1.0 EU per 1 μg of protein (determined by LAL method) |
| Biological Activity : | The ED50 for this effect is 1.4 ng/ml, measured in a cell proliferation assay using NIH-3T3 cell. |
| Activity Assay : | 1. Cell Line: NIH-3T3 (Mus mosculus/ embryo) 2. Maintenance Condition: RPMI 1640 containing 10% FBS 3. Assay Medium: serum free RPMI 1640 4. Cell density: 20,000 cells/well (96 well plate, final volume 100ul) 5. Serum Free Starvation: 24hr with RPMI1640 6. Incubation Time: 24 hr (after sample treatment) 7. Concentration Range: 1 pg/ml - 10 ug/ml (10-fold dilution)8. Detection method: BrdU assay |
| Storage : | Can be stored at +4℃ short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70℃. Avoid repeated freezing and thawing cycles. |
| Gene Name : | FGF21 fibroblast growth factor 21 [ Homo sapiens ] |
| Synonyms : | FGF21; fibroblast growth factor 21; FGF-21 |
| Gene ID : | 26291 |
| mRNA Refseq : | NM_019113 |
| Protein Refseq : | NP_061986 |
| UniProt ID : | Q9NSA1 |
| Chromosome Location : | 19q13.1-qter |
| MIM : | 609436 |
| Function : | growth factor activity; protein binding |
Products Types
| ◆ Recombinant Protein | ||
| FGF21-907H | Recombinant Human FGF21 Protein, His (Fc)-Avi-tagged | +Inquiry |
| Fgf21-931R | Recombinant Rat Fgf21 Protein, His-tagged | +Inquiry |
| FGF21-81H | Recombinant Active Human FGF21 Protein, His-tagged(C-ter) | +Inquiry |
| FGF21-85H | Recombinant Human FGF21 Protein | +Inquiry |
| FGF21-930H | Recombinant Human FGF21 Protein, His-tagged | +Inquiry |
| ◆ Lysates | ||
| FGF21-1890MCL | Recombinant Mouse FGF21 cell lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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