Recombinant Human FGF21, His-tagged

Cat.No. : FGF21-104H
Product Overview : Recombinant Human Fibroblast Growth Factor-21 produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 181 amino acids and an N-terminal Methionin (bold), having a molecular weight of 19.7 kDa (calculated). The amino acid sequence of the recombinant human FGF21 is 100% homologous to the amino acid sequence of the human FGF21 without signal sequence and contains 14 a. His-tag at N-terminal.
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Cat. No. : FGF21-104H
Description : FGF-21 is a unique member of FGF super family that is reported to be primarily produced by the liver. FGF-21 has recently been shown to have beneficial effects on a variety of metabolic parameters, including glucose and lipid metabolism and insulin sensitivity in animal models and is thought to exert potent antidiabetic and lipid-lowering agent of obesity and type 2 diabetes mellitus. FGF-21 activity depends on membrane protein beta Klotho that physically complexes with various FGF receptors, thus conferring them the ability to bind FGF-21 and activate downstream signaling pathways.
Source : Escherichia Coli.
Form : Liquid. In 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol
Molecular Weight : 21.6 kDa (202aa), confirmed by MALDI-TOF (molecular weight on SDS-PAGE will appear higher)
Purity : > 90% by SDS - PAGE
Concentration : 1 mg/ml (determined by Bradford assay)
Endotoxin Level : < 1.0 EU per 1 μg of protein (determined by LAL method)
Biological Activity : The ED50 for this effect is 1.4 ng/ml, measured in a cell proliferation assay using NIH-3T3 cell.
Activity Assay : 1. Cell Line: NIH-3T3 (Mus mosculus/ embryo) 2. Maintenance Condition: RPMI 1640 containing 10% FBS 3. Assay Medium: serum free RPMI 1640 4. Cell density: 20,000 cells/well (96 well plate, final volume 100ul) 5. Serum Free Starvation: 24hr with RPMI1640 6. Incubation Time: 24 hr (after sample treatment) 7. Concentration Range: 1 pg/ml - 10 ug/ml (10-fold dilution)8. Detection method: BrdU assay
Storage : Can be stored at +4℃ short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70℃. Avoid repeated freezing and thawing cycles.
Gene Name : FGF21 fibroblast growth factor 21 [ Homo sapiens ]
Synonyms : FGF21; fibroblast growth factor 21; FGF-21
Gene ID : 26291
mRNA Refseq : NM_019113
Protein Refseq : NP_061986
UniProt ID : Q9NSA1
Chromosome Location : 19q13.1-qter
MIM : 609436
Function : growth factor activity; protein binding

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.


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    The manufacturer's history of producing high-quality products contributed to our trust in this protein.


      Collaboration with other research groups was made easier by the product's standardized use in the scientific community.


        The product's role in promoting collaboration and teamwork positively impacted the quality of our research.

        Q&As (7)

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        How is FGF21 expression regulated in adipose tissue? 10/31/2022

        FGF21 expression in adipose tissue is regulated by various factors, including peroxisome proliferator-activated receptor gamma (PPARγ) activation.

        What are the effects of FGF21 on lipid metabolism? 08/27/2020

        FGF21 influences lipid metabolism by promoting the utilization of fatty acids for energy, contributing to the regulation of lipid homeostasis.

        Can FGF21 be a biomarker for metabolic health? 08/07/2019

        FGF21 levels are being explored as potential biomarkers for metabolic health, reflecting conditions such as insulin resistance and metabolic syndrome.

        Is FGF21 involved in the regulation of insulin sensitivity? 03/03/2019

        Yes, FGF21 plays a role in improving insulin sensitivity, making it a potential target for interventions in insulin resistance-associated conditions.

        Does FGF21 have roles in energy expenditure? 11/11/2018

        Yes, FGF21 influences energy expenditure by promoting thermogenesis in brown adipose tissue, contributing to overall metabolic regulation.

        Are there gender-specific differences in FGF21 levels? 08/30/2017

        Gender-specific differences in FGF21 levels have been reported, with some studies suggesting higher circulating FGF21 concentrations in females.

        What signaling pathways does FGF21 activate? 01/09/2016

        FGF21 activates signaling pathways such as the AMP-activated protein kinase (AMPK) pathway, which contributes to its metabolic effects.

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