Recombinant Mouse Fibroblast Growth Factor 21

• Cat.No.: Fgf21-109M
• Product Overview: Recombinant Mouse Fibroblast Growth Factor-21 produced inE.Coliis a single, non-glycosylated, polypeptide chain containing 183 amino acids including N-terminal Methionin and having a molecular mass of 20.1 kDa. The amino acid sequence of the recombinant mouse FGF21 is 100% homologous to the amino acid sequence of the Mouse FGF21 without signal sequence. The FGF-21 is purified by proprietary chromatographic techniques.

Specification

• Cat. No.: Fgf21-109M
• Description: FGF-21 is preferentially expressed in liver, but an exact knowledge of FGF-21 bioactivity and its mode of action have been lacking to date. FGF-21 is a potent activator of glucose uptake on adipocytes, protects animals from diet-induced obesity when overexpressed in transgenic mice, and lowers blood glucose and triglyceride levels when therapeutically administered to diabetic rodents.
• Source: Escherichia Coli.
• Amino Acid Sequence: MAYPIPDSSPLLQ FGGQVRQRYL YTDDDQDTEA HLEIREDGTV VGAAHRSPES LLELKALKPG VIQILGVKAS RFLCQQPDGA LYGSPHFDPE ACSFRELLLE DGYNVYQSEA HGLPLRLPQK DSPNQDATSW GPVRFLPMPG LLHEPQDQAG FLPPEPPDVG SSDPLSMVEP LQGRSPSYAS
• Physical Appearance: Sterile Filtered white lyophilized powder.
• Purity: Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
• Formulation: Filtered (0.4 µm) and lyophilized from 0.5 mg/ml in 20 mM TRIS, 20 mM NaCl, pH 7.5.
• Solubility: It is recommended to reconstitute the lyophilized Fibroblast Growth Factor-21 Mouse Recombinant in sterile 18 MΩ-cm H₂O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
• Stability: Recombinant Mouse Lyophilized FGF-21 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Fibroblast Growth Factor 21 should be stored at 4°Cbetween 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
• Tag: Non

Gene Information

• Gene Name: Fgf21 fibroblast growth factor 21 [ Mus musculus ]
• Synonyms: Fgf21; fibroblast growth factor 21
• Gene ID: 56636
• mRNA Refseq: NM_020013
• Protein Refseq: NP_064397
• UniProt ID: Q9JJN1
• Chromosome Location: 7 B2
• Pathway: MAPK signaling pathway; Melanoma; Pathways in cancer; Regulation of actin cytoskeleton
• Function: growth factor activity

Reviews

01/04/2022

The manufacturer's history of producing high-quality products contributed to our trust in this protein.

06/27/2021

Collaboration with other research groups was made easier by the product's standardized use in the scientific community.

03/03/2016

The product's role in promoting collaboration and teamwork positively impacted the quality of our research.

Q&As

How is FGF21 expression regulated in adipose tissue?

FGF21 expression in adipose tissue is regulated by various factors, including peroxisome proliferator-activated receptor gamma (PPARγ) activation.

What are the effects of FGF21 on lipid metabolism?

FGF21 influences lipid metabolism by promoting the utilization of fatty acids for energy, contributing to the regulation of lipid homeostasis.

Can FGF21 be a biomarker for metabolic health?

FGF21 levels are being explored as potential biomarkers for metabolic health, reflecting conditions such as insulin resistance and metabolic syndrome.

Is FGF21 involved in the regulation of insulin sensitivity?

Yes, FGF21 plays a role in improving insulin sensitivity, making it a potential target for interventions in insulin resistance-associated conditions.

Does FGF21 have roles in energy expenditure?

Yes, FGF21 influences energy expenditure by promoting thermogenesis in brown adipose tissue, contributing to overall metabolic regulation.

Are there gender-specific differences in FGF21 levels?

Gender-specific differences in FGF21 levels have been reported, with some studies suggesting higher circulating FGF21 concentrations in females.

What signaling pathways does FGF21 activate?

FGF21 activates signaling pathways such as the AMP-activated protein kinase (AMPK) pathway, which contributes to its metabolic effects.