| Description : |
Fibroblast growth factor 9 (FGF9) belongs to the large FGF family which has at least 23 members. All FGF family members are heparin binding growth factors with a core 120 amino acid (aa) FGF domain that allows for a common tertiary structure. FGF-9 targets glial cells, astrocytes cells and other cells that express the FGFR 1c, 2c, 3b, 3c, and 4. |
| Source : |
E. coli |
| Species : |
Human |
| Form : |
Sterile Filtered White lyophilized (freeze-dried) powder. |
| Bio-activity : |
Fully biologically active when compared to standard. The ED50 as determined by the dose-dependent stimulation of thymidine uptake by BaF3 cells expressing FGF receptors is ≤0.5 ng/mL, corresponding to a specific activity of ≥ 2 × 10^6 units/mg. |
| Molecular Mass : |
Approximately 23.4 kDa, a single non-glycosylated polypeptide chain containing 207 amino acids. |
| Protein Length : |
207 |
| AA Sequence : |
APLGEVGNYFGVQDAVPFGNVPVLP VDSPVLLSDHLGQSEAGGLPRGPAV TDLDHLKGILRRRQLYCRTGFHLEI FPNGTIQGTRKDHSRFGILEFISIA VGLVSIRGVDSGLYLGMNEKGELYG SEKLTQECVFREQFEENWYNTYSSN LYKHVDTGRRYYVALNKDGTPREGT RTKRHQKFTHFLPRPVDPDKVPELY KDILSQS |
| Endotoxin : |
Less than 1 EU/mg of rHuFGF-9 as determined by LAL method. |
| Purity : |
>95% by SDS-PAGE and HPLC analyses. |
| Storage : |
This lyophilized preparation is stable for several weeks at 2-8 centigrade, but should be kept at -20 centigrade for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 centigrade. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 to -70 centigrade. Avoid repeated freeze/thaw cycles. |
| Storage Buffer : |
Lyophilized from a 0.2mm filtered concentrated solution in PBS, pH 7.4. |
| Reconstitution : |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at < -20 centigrade. Further dilutions should be made in appropriate buffered solutions. |
