| Species : |
Rat |
| Source : |
E.coli |
| Tag : |
Non |
| Description : |
Fibroblast growth factor 9 (FGF-9) belongs to the large FGF family which has at least 23 members. All FGF family members are heparin binding growth factors with a core 120 amino acid (a.a.) FGF domain that allows for a common tertiary structure. FGF-9 targets glial cells, astrocytes cells and other cells that express the FGFR 1c, 2c, 3b, 3c, and 4. |
| Form : |
Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM Tris, 400 mM NaCl, pH8.0. |
| Bio-activity : |
Fully biologically active when compared to standard. The ED 50 determined by a cell proliferation assay using murine Balb/c 3T3 cells is less than 1 ng/ml, corresponding to a specific activity of >1.0 × 10^6 IU/mg. |
| Molecular Mass : |
23.1 kDa |
| AA Sequence : |
LGEVGSYFGV QDAVPFGNVP VLPVDSPVLL SDHLGQSEAG GLPRGPAVTD LDHLKGILRR RQLYCRTGFH LEIFPNGTIQ GTRKDHSRFG ILEFISIAVG LVSIRGVDSG LYLGMNEKGE LYGSEKLTQE CVFREQFEEN WYNTYSSNLY KHVDTGRRYY VALNKDGTPR EGTRTKRHQK FTHFLPRPVD PDKVPELYKD ILSQS |
| Endotoxin : |
Less than 1 EU/μg of rRtFGF-9-1 as determined by LAL method. |
| Purity : |
>95% by SDS-PAGE and HPLC analyses. |
| Usage : |
This material is for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE. |
| Storage : |
This lyophilized preparation is stable at 2-8 centigrade, but should be kept at -20 centigrade for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 centigrade. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 centigrade to -70 centigrade. Avoid repeated freeze/thaw cycles. |
| Reconstitution : |
We recommend that this vial be briefy centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at< -20="" centigrade.="" further="" dilutions="" should="" be="" made="" in="" appropriate="" buffered="" solutions.=""> |
