Recombinant Human FK506 Binding Protein 14, 22 kDa, His-tagged

• Cat.No.: FKBP14-2574H
• Product Overview: Recombinant human FKBP14 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography. MW = 24.2 kDa (213aa).

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: FKBP14, also known as 22 kDa FK506-binding protein, is an enzyme that accelerates the folding of proteins during protein synthesis. This protein contains two EF-hand domains and one PPIase FKBP-type domain. Truncation of the amino-terminus of FKBP14 greatly reduces peptidyl prolyl cis-trans isomerase activity, therefore suggesting that the PPIase FKBP-type domain must be located at the N-terminus.
• Sequences Of Amino Acids: MGSSHHHHHH SSGLVPRGSH MALIPEPEVK IEVLQKPFIC HRKTKGGDLM LVHYEGYLEK DGSLFHSTHK HNNGQPIWFT LGILEALKGW DQGLKGMCVG EKRKLIIPPA LGYGKEGKGK IPPESTLIFN IDLLEIRNGP RSHESFQEMD LNDDWKLSKD EVKAYLKKEF EKHGAVVNES HHDALVEDIF DKEDEDKDGF ISAREFTYKH DEL
• Purity: > 90% by SDS – PAGE.
• Form: Liquid in Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol.
• Concentration: 1 mg/ml (determined by Bradford assay).
• Biological activity: Specific activity is > 240 nmoles/min/mg, and is defined as the amount of enzyme that cleaves 1umole of suc-AAFP-pNA per minute at 25°C in Tris-Hcl pH8.0 using chymotrypsin.
• Activity Assay: 1. Prepare 170ul assay buffer into a suitable container and pre-chill on ice before use: The final concentrations are 200 mM Tris-Hcl, pH 8.0, and 20nM chymotrypsin.2. Add 10ul of recombinant FKBP14 protein with 1ug in assay buffer. 3. Mix by inversion and equilibrate to 1°C and monitor the A405nm until the value is constant using a spectrophotometer.4. Add 20ul pre-chilled 5mM suc-AAFP-pNA. (Substrate was dissolved in TFE that contained 460mM LiCl to a concentration of 3 mM)5. Record the increase in A405 nm for 30 minutes at 25°C.
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: FKBP14 FK506 binding protein 14, 22 kDa [ Homo sapiens ]
• Synonyms: FKBP14; FK506 binding protein 14, 22 kDa; FKBP22; FLJ20731; peptidyl-prolyl cis-trans isomerase FKBP14; FKBP-14; FKBP-22; rotamase; 22 kDa FKBP; PPIase FKBP14; FK506-binding protein 14; 22 kDa FK506-binding protein; EC 5.2.1.8; FK506 binding protein 14 (22 kDa)
• Gene ID: 55033
• mRNA Refseq: NM_017946
• Protein Refseq: NP_060416
• UniProt ID: Q9NWM8
• Chromosome Location: 7p14.3
• Pathway: Diabetes pathways
• Function: calcium ion binding; isomerase activity; peptidyl-prolyl cis-trans isomerase activity