Recombinant Human FLT1, His-tagged

• Cat.No.: FLT1-544H
• Product Overview: Recombinant human FLT1 was expressed by baculovirus inSf9 cells usingan N-terminal His tag. MW=67.5kDa.

Specification

• Species: Human
• Source: Sf9 Cells
• Tag: His
• Description: FLT1, also known as fms-like tyrosine kinase 1, is a member of the VEGFR family and is related to the oncogene ROS. The tyrosine kinase activity of FLT1 has been shown to play a key role in control of cell proliferation and differentiation. FLT1 is a receptor for vascular endothelial growth factor (VEGF) and it has been observed that the ratio of FLT1 mRNA to VEGF mRNA correlates with tumor angiogenesis and prognosis in non-small cell lung cancers. In addition to the full-length receptor, FLT1 gene also encodes a soluble form of receptor due to alternative splicing. Both the full length and soluble form of FLT1 show strong binding affinity for VEGF.
• Purity: >70% by SDS-PAGE.
• Specific Activity: 170 U/mg. One unit is defined as the amount of enzyme that will transfer 1 nmol phosphate to the tyrosine substrate per minute at pH 7.4 and 30°C. Assay buffer: 50 mM HEPES, pH 7.4, 3 mM MgCl2, 3 mM MnCl2, 1 mM DTT, 3 uM Na-orthovanadate, 0.1 mM ATP, 30 ug/ml Poly (Glu:Tyr)4:1 substrate, and 2 ug /ml recombinant Flt1.
• Application: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
• Formulated In: 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 50% glycerol, and 3 mM DTT.
• Stability: >6 months at –80°C.

Gene Information

• Gene Name: FLT1 fms-related tyrosine kinase 1 (vascular endothelial growth factor/vascular permeability factor receptor) [ Homo sapiens ]
• Synonyms: FLT1; fms-related tyrosine kinase 1 (vascular endothelial growth factor/vascular permeability factor receptor); FLT; VEGFR1; vascular endothelial growth factor receptor 1; EC 2.7.10.1; VEGFR-1; Vascular permeability factor receptor; Flt-1; Tyrosine-protein kinase receptor FLT; Tyrosine-protein kinase FRT; Fms-like tyrosine kinase 1; FRT
• Gene ID: 2321
• mRNA Refseq: NM_001159920
• Protein Refseq: NP_001153392
• MIM: 165070
• UniProt ID: P17948
• Chromosome Location: 13q12
• Pathway: Cytokine-cytokine receptor interaction; Endocytosis; Focal adhesion; Signaling by VEGF
• Function: ATP binding; growth factor binding; identical protein binding; nucleotide binding; receptor activity; transferase activity; vascular endothelial growth factor receptor activity