Recombinant Human FLT3LG protein, C-His-tagged FLT3LG-156H

Recombinant Human FLT3LG protein, C-His-tagged

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Recombinant Human FLT3LG protein, C-His-tagged

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Cat.No. : FLT3LG-156H
Product Overview : Recombinant Human FLT3LG protein (Thr27-Pro185) was fused to His-tag at the C-terminus and expressed in Baculovirus-Insect cells.
Description : FMS-like tyrosine kinase 3 ligand (Flt-3 Ligand) is also known as FL, Flt3L and FLT3LG, is an α-helical cytokine that promotes the differentiation of multiple hematopoietic cell lineages. FLT3LG is expressed as a noncovalentlylinked dimer by T cells and bone marrow and thymic fibroblasts. Each 36 kDa chain carries approximately 12 kDa of N- and O- linked carbohydrates. FLT3LG is structurally homologous to stem cell factor (SCF) and colony stimulating facor 1 (CSF-1). FLT3LG acts as a growth factor that increases the number of immune cells by activating the hematopoietic progenitors. It also induces the mobilization of the hematopoietic progenitors and stem cells in vivo which may help the system to kill cancer cells. FLT3LG induces the expansion of monocytes and immature dendritic cells as well as early B cell lineage differentiation. FLT3LG cooperates with IL2, IL6, IL7, and IL15 to induce NK cell development and with IL3, IL7 and IL11 to induce terminal B cell maturation. Animal studies also show FLT3LG to reduce the severity of experimentally induced allergic inflammation. FLT3LG is crucial for steady-state pDC and cDC development. A lack of FLT3L results in low levels of DCs.
Source : Baculovirus-Insect cells
Species : Human
Tag : His
Predicted N Terminal : Thr27
Form : Lyophilized from 0.22 μm filtered solution in PBS, pH7.4, 10% trehalose.
Molecular Mass : The protein has a calculated MW of 19.9 kDa. The protein migrates as 22-28 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Protein length : Thr27-Pro185
Endotoxin : Less than 1.0 EU per μg by the LAL method.
Purity : >90% as determined by SDS-PAGE.
Storage : For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4°C before opening to recover the entire contents.
SDS-PAGE of FLT3LG-156H :

Human Flt-3 Ligand, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

Bioactivity-ELISA of FLT3LG-156H :

Immobilized Human Flt-3, Fc Tag at 5 μg/mL (100 μL/well) can bind Human Flt-3 Ligand, His Tag with a linear range of 0.1-0.6 ng/mL (QC tested).

Gene Name : FLT3LG
Official Symbol : FLT3LG
Synonyms : FL; FLG3L; FLT3L
Gene ID : 2323
mRNA Refseq : NM_001204502.2
Protein Refseq : NP_001191431.1
MIM : 600007
UniProt ID : P49771

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