Recombinant Human GRK5 protein(Met1-Ser590), His-tagged

• Cat.No.: GRK5-760H
• Product Overview: Recombinant Human GRK5 (NP_005299.1) (Met 1-Ser 590) was expressed in Insect Cells, fused with a polyhistidine tag at the C-terminus.

Specification

• Source: Insect Cells
• Species: Human
• Tag: C-His
• Protein length: Met1-Ser590
• Form: Lyophilized from sterile 50mM Tris, 100mM NaCl, 0.5mM PMSF, 1mM DTT, 0.5mM EDTA, 10% gly, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Molecular Mass: The recombinant human GRK5 consists of 600 amino acids and predicts a molecular mass of 69 kDa. The apparent molecular mass of rhGRK5 is approximately 58 kDa in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: > 90 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: GRK5 G protein-coupled receptor kinase 5 [ Homo sapiens ]
• Official Symbol: GRK5
• Synonyms: GRK5; G protein-coupled receptor kinase 5; GPRK5; FP2025; g protein-coupled receptor kinase GRK5; FLJ39780
• Gene ID: 2869
• mRNA Refseq: NM_005308
• Protein Refseq: NP_005299
• MIM: 600870
• UniProt ID: P34947