Recombinant Human Growth Differentiation Factor 11

• Cat.No.: GDF11-242
• Product Overview: Recombinant human GDF-11 produced inE.coliis a 25.0 kDa disulfide-linked homodimer containing two 109 amino acid polypeptide chains.

Specification

• Species: Human
• Source: E.coli
• Tag: Non
• Description: GDF-11 is a myostatin-homologous protein that acts as an inhibitor of nerve tissue growth. GDF-11 has been shown to suppress neurogenesis through a myostatin-like pathway, which involves arrest of progenitor cell-cycle in the G1 phase. Similarities between myostatin and GDF-11, which are 90% identical in their amino acid sequence, suggests that the regulatory mechanisms responsible for maintaining proper tissue size during neural and muscular development might be the same.
• Purity: > 98% by SDS-PAGE and HPLC analysis.
• Endotoxin level: < 0.1 ng per μg of Galectin-1.
• Stabilizer: None.
• Formulation: Lyophilized.
• Biologically Activity: The ED50 was determined by its ability to inhibit induced alkaline phosphatase production by ATDC-5 chondrogenic cells. The expected ED50 for this effect is 0.08-0.10 μg/ml.
• Reconstitution: The human GDF-11 should be reconstituted in water to a concentration of 0.1-1.0 mg/ml. This solution can be diluted into other aqueous buffers and stored at 4°Cfor one week or -20°Cfor future use.
• Stability: The lyophilized protein is stable for at least 2 years from date of receipt at -20°C. Reconstituted GDF-11 is stable for at least 3 months when stored in working aliquots with a carrier protein at -20°C. Avoid repeated freeze-thaw cycles.

Gene Information

• Gene Name: GDF11 growth differentiation factor 11 [ Homo sapiens ]
• Synonyms: GDF11; growth differentiation factor 11; BMP11; BMP-11; bone morphogenetic protein 11; GDF-11
• Gene ID: 10220
• mRNA Refseq: NM_005811
• Protein Refseq: NP_005802
• MIM: 603936
• UniProt ID: O95390
• Chromosome Location: 12q13.13
• Function: cytokine activity; growth factor activity; protein binding