Recombinant Human HRAS

• Cat.No.: HRAS-28759TH
• Product Overview: Recombinant full length, proprietary tagged, Human H-Ras protein. 189 amino acids; Predicted MWt 47 kDa.

Specification

• Description: This gene belongs to the Ras oncogene family, whose members are related to the transforming genes of mammalian sarcoma retroviruses. The products encoded by these genes function in signal transduction pathways. These proteins can bind GTP and GDP, and they have intrinsic GTPase activity. This protein undergoes a continuous cycle of de- and re-palmitoylation, which regulates its rapid exchange between the plasma membrane and the Golgi apparatus. Mutations in this gene cause Costello syndrome, a disease characterized by increased growth at the prenatal stage, growth deficiency at the postnatal stage, predisposition to tumor formation, mental retardation, skin and musculoskeletal abnormalities, distinctive facial appearance and cardiovascular abnormalities. Defects in this gene are implicated in a variety of cancers, including bladder cancer, follicular thyroid cancer, and oral squamous cell carcinoma. Multiple transcript variants, which encode different isoforms, have been identified for this gene.
• Source: E. coli
• Biological activity: HRAS-28759TH is effective in activation of PI3K and PKB
• Form: Liquid
• Purity: >95% by SDS-PAGE
• Storage buffer: Preservative: NoneConstituents: 5mM Magnesium chloride, 5mM Beta mercaptoethanol, 25mM Tris HCl, 150mM Sodium chloride, pH 7.5
• Storage: Shipped on dry ice. Upon delivery aliquot and store at -80oC. Avoid freeze / thaw cycles.

Gene Information

• Gene Name: HRAS v-Ha-ras Harvey rat sarcoma viral oncogene homolog [ Homo sapiens ]
• Official Symbol: HRAS
• Synonyms: HRAS; v-Ha-ras Harvey rat sarcoma viral oncogene homolog; HRAS1; GTPase HRas
• Gene ID: 3265
• mRNA Refseq: NM_001130442
• Protein Refseq: NP_001123914
• MIM: 190020
• Uniprot ID: P01112
• Chromosome Location: 11p15.5
• Pathway: ARMS-mediated activation, organism-specific biosystem; Activation of NMDA receptor upon glutamate binding and postsynaptic events, organism-specific biosystem; Acute myeloid leukemia, organism-specific biosystem; Acute myeloid leukemia, conserved biosystem; Axon guidance, organism-specific biosystem
• Function: GTP binding; GTPase activity; Ral guanyl-nucleotide exchange factor activity; nucleotide binding; protein C-terminus binding