Recombinant Human HRAS ΔC (S17N) Protein

• Cat.No.: HRAS-3848H
• Product Overview: Recombinant Human HRAS ΔC (S17N) Protein is produced by E. coli expression system. HRAS ΔC (S17N) (aa 1-171) lacks the C-terminus with the CaaX recognition sequence necessary for anchoring Ras into the plasma membrane. The mutation S17N results in a 40-fold increase in the affinity for GTP without affecting its affinity for GDP.

Specification

• Description: Ras proteins are members of the superfamily of small GTP-binding proteins that function as molecular switches controlling a variety of signaling and transport pathways. H-Ras is one of the classic human Ras proteins (H-, N-, K-Ras4A, and K-Ras4B). H-Ras ΔC (aa 1 - 171) lacks the C-terminus with the CaaX recognition sequence necessary for anchoring Ras into the plasma membrane. The mutation S17N results in a 40-fold increase in the affinity for GTP without affecting its affinity for GDP. Protein preparation is 95% GDP- and 5% GTP-loaded, measured by HPLC.
• Source: E. coli
• Species: Human
• Form: liquid (Supplied in 64 mM Tris-HCl pH 7.2, 10 mM MgCl2 and 5 mM DTE)
• Molecular Mass: 19.5 kDa
• Protein length: 171 amino acids
• Purity: > 95 % (SDS-PAGE)
• Notes: For in vitro use only!
• Storage: Store at -80 centigrade.
  Avoid freeze/thaw cycles.
• Shipping: Shipped on dry ice.

Gene Information

• Gene Name: HRAS HRas proto-oncogene, GTPase [ Homo sapiens (human) ]
• Official Symbol: HRAS
• Synonyms: CTLO; KRAS; HAMSV; HRAS1; KRAS2; RASH1; RASK2; Ki-Ras; p21ras; C-H-RAS; c-K-ras; H-RASIDX; c-Ki-ras; C-BAS/HAS; C-HA-RAS1
• Gene ID: 3265
• mRNA Refseq: NM_005343
• Protein Refseq: NP_005334
• MIM: 190020
• UniProt ID: P01112