Active Recombinant Human IL17A Protein

• Cat.No.: IL17A-102H
• Product Overview: Recombinant Human Interleukin-17A is produced by our E.coli expression system and the target gene encoding Ile20-Ala155 is expressed.

Specification

• Description: Interleukin-17 is a potent pro-inflammatory cytokine produced by activated memory T cells. There are at least six members of the IL-17 family in humans and in mice. As IL-17 shares properties with IL-1 and TNF-alpha, it may induce joint inflammation and bone and cartilage destruction. This cytokine is found in synovial fluids of patients with rheumatoid arthritis, and produced by rheumatoid arthritis synovium. It increases IL-6 production, induces collagen degradation and decreases collagen synthesis by synovium and cartilage and proteoglycan synthesis in cartilage. IL-17 is also able to increase bone destruction and reduce its formation. Blocking of interleukin-17 with specific inhibitors provides a protective inhibition of cartilage and bone degradation.
• Source: E. coli
• Species: Human
• Form: Lyophilized from a 0.2 um filtered solution of 20mM PB, pH 7.4.
• Bio-activity: ED50 is approximately 2 ng/ml. Specific Activity of 5 x 10^5 IU/mg. Measured by the dose-dependent induction of IL-6 in primary human foreskin fibroblasts.
• Protein Length: Ile20-Ala155
• AA Sequence: MIVKAGITIPRNPGCPNSEDKNFPR TVMVNLNIHNRNTNTNPKRSSDYYN RSTSPWNLHRNEDPERY PSVIWEAKCRHLGCIN ADGNVDYHMNSVPIQQEILVLRREP PHCPNSFRLEKILVSVGCTCVTPIV HHVA
• Endotoxin: Less than 0.1 ng/ug (1 EU/ug).
• Purity: >95%
• Storage: Lyophilized protein should be stored at < -20 centigrade, though stable at room temperature for 3 weeks.
  Reconstituted protein solution can be stored at 4-7 centigrade for 2-7 days.
  Aliquots of reconstituted samples are stable at < -20 centigrade for 3 months.
• Quality Statement: Purity: Greater than 95% as determined by reducing SDS-PAGE.
• Shipping: The product is shipped at ambient temperature.
  Upon receipt, store it immediately at the temperature listed below.

Gene Information

• Gene Name: IL17A interleukin 17A [ Homo sapiens (human) ]
• Official Symbol: IL17A
• Synonyms: Interleukin-17A; IL-17; IL-17A; Cytotoxic T-Lymphocyte-Associated Antigen 8; CTLA-8; IL17A
• Gene ID: 3605
• mRNA Refseq: NM_002190.3
• Protein Refseq: NP_002181.1
• MIM: 603149
• UniProt ID: Q16552

Reviews

12/16/2018

Good for receptor studies.

03/05/2018

Effective in Th17 differentiation.

03/08/2017

High bioactivity in various tissue types.

Q&As

Could you explain the role of IL17A in autoimmune disease progression using animal models?

Animal models, such as experimental autoimmune encephalomyelitis, explore IL17A's role in autoimmunity.

Can you detail the protocols for inducing IL17A expression in T cells in vitro?

Inducing IL17A expression in T cells involves culturing them with specific cytokines.

What strategies elucidate IL17A's impact on tissue-specific inflammation in human samples?

Studying IL17A's influence on tissue-specific inflammation in humans involves analyzing patient samples.

Can you elaborate on the use of neutralizing antibodies in blocking IL17A's effects?

Neutralizing antibodies block IL17A's effects in cell cultures or animal models.

How does one perform a multiplex cytokine assay to quantify IL17A along with other cytokines?

Multiplex cytokine assays use fluorescent beads to measure IL17A and other cytokines simultaneously.

How is the IL17A receptor (IL17RA) signaling pathway activated and studied?

IL17RA signaling is triggered by IL17A binding and is studied through downstream phosphorylation assays.

What are the methods to investigate the impact of IL17A on epithelial barrier integrity?

Methods like transepithelial electrical resistance (TEER) assay evaluate IL17A's effect on barrier function.

How is IL17A's contribution to neutrophil recruitment assessed in inflammatory models?

Inflammatory models, like airway inflammation assays, examine IL17A's impact on neutrophil recruitment.

What techniques are used to analyze IL17A-producing cell populations within a tissue?

Flow cytometry or intracellular staining help identify IL17A-producing cell subsets within tissues.

What experimental design is employed to understand IL17A's involvement in cancer development?

Experimental designs combine cancer cell lines with IL17A stimulation to investigate its pro-tumor effects.