| Cat.No. : |
IL1F7-318H
Optional Service: Optional requirements on this protein
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| Product Overview : |
Recombinant Human IL-1F7 (Lys27-Asp192) produced in E. coli is a single, non-glycosylated, polypeptide chain containing 167 amino acids and having a molecular mass of 18.6 kDa. |
| Description : |
Human interleukin family 1, member 7 (hIL-1F7 / FIL1-zeta) is a member of the interleukin 1 cytokine family. Five alternatively spliced transcript variants encoding distinct isoforms have been reported with distinct expression profiles. The longest IL-1F7 transcript, referred to as IL1F7b or IL1F7 isoform 1, encodes a 218 a.a. residues proprotein containing a 45 a.a. propeptide which is cleaved to generate mature protein. IL-1F7b binds to IL-18 Rα with low affinity but does not exert any IL-18 agonistic or antagonistic effects. IL-1F7b also binds interleukin 18 binding protein (IL-18BP), an inhibitory binding protein of interleukin 18 (IL-18), and subsequently forms a complex with IL-18 receptor beta subunit, and through which it inhibits the activity of IL-18. |
| Biological Activity : |
As measured by its binding ability in a functional ELISA, immobilized rHuIL-1F7 at 1 μg/ml (100 μl/well) can bind rhIL-18 R/Fc Chimera with a linear range of 0.015~1 μg/mL. |
| Purity : |
Greater than 95% as determined by (a) Analysis by RP-HPLC; (b) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel. |
| Amino-Acid Sequence : |
The sequence of the first five N-terminal amino acids was determined and was found to be Met-Lys-Asn-Leu-Asn. |
| Endotoxin : |
Less than 0.1ng/µg (1 IEU/µg) determined by LAL test. |
| Form : |
The protein was lyophilized after extensive dialysis against 20mM Phosphate buffer, pH7.4. |
| Reconstitution : |
It is recommended to quick spin followed by reconstitution of rHuIL-1F7 in PBS to a concentration no less than 100µg/ml, which can then be further diluted to other aqueous solutions. |
| Storage : |
Lyophilized rHuIL-1F5 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution rHuIL-1F7 should be stored at 4°C between 2-7 days and for future use below -18°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles. |
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
