Recombinant Human Insulin Receptor, Fc Chimera

Cat.No. : INSR-611H
  • Specification
  • Gene Information
  • Related Products
  • Citation
  • Download
Source : HEK293
Species : Human
Tag : Fc
Form : PBS, pH 7.4
Protein length : 1151 amino acids
AA Sequence : MDIRNNLTRLHELENCSVIEGHLQI LLMFKTRPEDFRDLSFPKLIMITDY LLLFRVYGLESLKDLFPNLTVIRGS RLFFNYALVIFEMVHLKELGLYNLM NITRGSVRIEKNNELCYLATIDWSR ILDSVEDNYIVLNKDDNEECGDICP GTAKGKTNCPATVINGQFVERCWTH SHCQKVCPTICKSHGCTAEGLCCHS ECLGNCSQPDDPTKCVACRNFYLDG RCVETCPPPYYHFQDWRCVNFSFCQ DLHHKCKNSRRQGCHQYVIHNNKCI PECPSGYTMNSSNLLCTPCLGPCPK VCHLLEGEKTIDSVTSAQELRGCTV INGSLIINIRGGNNLAAELEANLGL IEEISGYLKIRRSYALVSLSFFRKL RLIRGETLEIGNYSFYALDNQNLRQ LWDWSKHNLTITQGKLFFHYNPKLC LSEIHKMEEVSGTKGRQERNDIALK TNGDQASCENELLKFSYIRTSFDKI LLRWEPYWPPDFRDLLGFMLFYKEA PYQNVTEFDGQDACGSNSWTVVDID PPLRSNDPKSQNHPGWLMRGLKPWT QYAIFVKTLVTFSDERRTYGAKSDI IYVQTDATNPSVPLDPISVSNSSSQ IILKWKPPSDPNGNITHYLVFWERQ AEDSELFELDYCLKGLKLPSRTWSP PFESEDSQKHNQSEYEDSAGECCSC PKTDSQILKELEESSFRKTFEDYLH NVVFVPRKTSSGTGAEDPRPSRKRR SLGDVGNVTVAVPTVAAFPNTSSTS VPTSPEEHRPFEKVVNKESLVISGL RHFTGYRIELQACNQDTPEERCSVA AYVSARTMPEAKADDIVGPVTHEIF ENNVVHLMWQEPKEPNGLIVLYEVS YRRYGDEELHLCVSRKHFALERGCR LRGLSPGNYSVRIRATSLAGNGSWT EPTYFYVTDYLDVPSNIAKIEGRMD PKSSDKTHTCPPCPAPELLGGPSVF LFPPKPKDTLMISRTPEVTCVVVDV SHEDPEVKFNWYVDGVEVHNAKTKP REEQYNSTYRVVSVLTVLHQDWLNG KEYKCKVSNKALPAPIEKTISKAKG QPREPQVYTLPPSRDELTKNQVSLT CLVKGFYPSDIAVEWESNGQPENNY KTTPPVLDSDGSFFLYSKLTVDKSR WQQGNVFSCSVMHEALHNHYTQKSL SLSPGK
Endotoxin : Less than 1.0 EU per μg by the LAL method.
Purity : >80% by SDS-PAGE
Storage : Store at -20 ~ -80 centigrade. Avoid repeated freeze-thaw cycles.
Gene Name : INSR insulin receptor [Homo sapiens]
Official Symbol : INSR
Synonyms : INSR; insulin receptor; HHF5; CD220; EC 2.7.10.1; IR; CD220 antigen; Insulin receptor subunit alpha; Insulin receptor subunit beta
Gene ID : 3643
mRNA Refseq : NM_000208
Protein Refseq : NP_000199
MIM : 147670
UniProt ID : P06213

Suppression of the Insulin Receptors in Adult Schistosoma japonicum Impacts on Parasite Growth and Development: Further Evidence of Vaccine Potential

Journal: PLoS Neglected Tropical Diseases    PubMed ID: 25961574    Data: 2015/5/11

Authors: Hong You, Geoffrey N. Gobert, Mark Pearson

Article Snippet:Recombinant human insulin receptor (HIR) (short isoform) extracellular domain (Met1-Lys 956) (Creative BioMart, Inc., USA) and recombinant SjLD1 and SjLD2 (expressed in E . coli ) [ ] (see below) were subjected to electrophoresis on 12% (w/v) SDS-PAGE gels (1μg in 10μl SDS-PAGE sample buffer) as described above and then blotted onto a nitrocellulose membrane.. Rabbit anti-HIR polyclonal antibody (1:300) (Creative BioMart, Inc, Shirley, NY, USA), produced in a rabbit immunized with purified human cell-derived recombinant HIR extracellular domain (Met1-Lys 956), and the rabbit anti-SjLD1 (1:50) and anti-SjLD2 (1:50) sera were used as primary antibodies.Rabbit anti-HIR polyclonal antibody (1:300) (Creative BioMart, Inc, Shirley, NY, USA), produced in a rabbit immunized with purified human cell-derived recombinant HIR extracellular domain (Met1-Lys 956), and the rabbit anti-SjLD1 (1:50) and anti-SjLD2 (1:50) sera were used as primary antibodies.

Results are shown for proteins recognised by anti-Sm-Pmy antibody (top panel), anti-SjLD1 (middle panel) and anti-SjLD2 (bottom panel) antibodies. The intensity of Sm-Pmy expression was evaluated so as to determine equal protein loading. The arrows indicate the diminished level of SjIR proteins relative to the luciferase treatment control in the first lane. The experiment was repeated twice with similar results obtained. B . Western blot analysis showing no immunological cross reactivity between recombinant HIR and the SjLDs. Commercial recombinant human insulin receptor (rHIR) and recombinant SjLD1 (rSjLD1) and SjLD2 (rSjLD2) were electrophoresed on SDS-PAGE gels, blotted to membrane and probed with rabbit anti-HIR polyclonal antibody (left panel), rabbit anti-SjLD1 (middle panel) and anti-SjLD2 (right panel) as primary antibodies with anti-rabbit IgG conjugated to horseradish peroxidise used as secondary antibody.

Results are shown for proteins recognised by anti-Sm-Pmy antibody (top panel), anti-SjLD1 (middle panel) and anti-SjLD2 (bottom panel) antibodies. The intensity of Sm-Pmy expression was evaluated so as to determine equal protein loading. The arrows indicate the diminished level of SjIR proteins relative to the luciferase treatment control in the first lane. The experiment was repeated twice with similar results obtained. B . Western blot analysis showing no immunological cross reactivity between recombinant HIR and the SjLDs. Commercial recombinant human insulin receptor (rHIR) and recombinant SjLD1 (rSjLD1) and SjLD2 (rSjLD2) were electrophoresed on SDS-PAGE gels, blotted to membrane and probed with rabbit anti-HIR polyclonal antibody (left panel), rabbit anti-SjLD1 (middle panel) and anti-SjLD2 (right panel) as primary antibodies with anti-rabbit IgG conjugated to horseradish peroxidise used as secondary antibody.

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

Inquiry

  • Reviews
  • Q&As

Customer Reviews (3)

Write a review
Reviews
02/25/2020

    The compatibility with a range of lab equipment streamlined experimental procedures, saving both time and effort.

    04/26/2016

      The product's support in the form of educational resources positively impacted the learning curve for our researchers.

      04/18/2016

        The proactive engagement with customer insights contributed to the product's ongoing relevance in the scientific community.

        Q&As (7)

        Ask a question
        What impact does malfunction or dysregulation of the insulin receptor (INSR) have on metabolic health? 10/31/2020

        Malfunction or dysregulation of the insulin receptor (INSR) can lead to disturbances in metabolic health, contributing to conditions like diabetes.

        How does the insulin receptor (INSR) function in regulating glucose metabolism in cells? 04/08/2020

        INSR regulates glucose metabolism by facilitating the cellular uptake of glucose in response to insulin.

        What are the key tissues or cell types where the insulin receptor (INSR) is prominently expressed? 08/29/2019

        The insulin receptor (INSR) is prominently expressed in tissues such as the liver, muscle, and adipose tissue.

        Are there known genetic variations or mutations in the insulin receptor (INSR) associated with metabolic disorders or insulin resistance? 02/27/2019

        Genetic variations or mutations in the insulin receptor (INSR) are associated with certain metabolic disorders and insulin resistance.

        In the context of diabetes, what role does the insulin receptor (INSR) play in the development of insulin resistance? 06/16/2018

        In diabetes, the insulin receptor (INSR) contributes to the development of insulin resistance, impairing its normal signaling functions.

        What is the primary role of the insulin receptor (INSR) protein in cellular processes? 04/29/2017

        The insulin receptor (INSR) protein plays a crucial role in cellular processes, particularly in mediating the effects of insulin.

        How does the insulin receptor (INSR) activate downstream signaling pathways in response to insulin binding? 09/14/2016

        Upon insulin binding, the insulin receptor (INSR) activates downstream signaling pathways, including the PI3K-AKT pathway.

        Ask a Question for All INSR Products

        Required fields are marked with *

        My Review for All INSR Products

        Required fields are marked with *

        0

        Inquiry Basket

        cartIcon
        logo

        FOLLOW US

        Terms and Conditions        Privacy Policy

        Copyright © 2024 Creative BioMart. All Rights Reserved.

        Contact Us

        • /
        • Service lnquiry:

        Stay Updated on the Latest Bioscience Trends