Active Recombinant Human INSR, His-tagged

• Cat.No.: INSR-661H
• Product Overview: Recombinant Human INSR (Accession # NP_001073285) His28-Arg750 ( alpha subunit) & Ser751-Lys944, fused with a C-terminal 10-His tag ( beta subunit), was produced in Mouse myeloma cell line, NS0-derived.

Specification

• Species: Human
• Source: Mammalian Cells
• Tag: His
• Protein Length: 28-750;751-944 a.a.
• Predicted N Terminal: His28 ( alpha subunit) & Ser751( beta subunit)
• Form: Lyophilized from a 0.2 µm filtered solution in PBS with BSA as a carrier protein.
• Bio-activity: Measured by its binding ability in a functional ELISA.When 15 ng/mL of biotinylated recombinant human Insulin is added to serially diluted Recombinant Human Insulin R/CD220, the concentration of Recombinant Human Insulin R/CD220 that produces 50% of the optimal binding response is typically 0.03-0.15 µg/mL.
• Molecular Mass: Recombinant Human INSR, His-tagged has a calculated MW of 82.9 kDa ( alpha subunit), 22.9 kDa ( beta subunit). In SDS-PAGE migrates as 122-135 kDa and 33-43 kDa, reducing conditions.
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
• Reconstitution: Reconstitute at 100 µg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Gene Information

• Gene Name: INSR insulin receptor [ Homo sapiens ]
• Official Symbol: INSR
• Synonyms: INSR; insulin receptor; CD220; IR; HHF5
• Gene ID: 3643
• mRNA Refseq: NM_000208
• Protein Refseq: NP_000199
• MIM: 147670
• UniProt ID: P06213
• Chromosome Location: 19p13.3-p13.2