Active Recombinant Human IL12B protein(Met1-Ser328), His-tagged
Cat.No. : | IL12B-145H |
Product Overview : | Recombinant Human IL12, termed as IL12B (NP_002178.2) (Met 1-Ser 328) was expressed in HEK293, fused with a polyhistidine tag at the C-terminus. |
Availability | July 26, 2024 |
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Source : | HEK293 |
Species : | Human |
Tag : | His |
Protein length : | Met1-Ser328 |
Form : | Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. |
Bio-activity : | Measured by its ability to bind biotinylated recombinant human IL12RB1 in a functional ELISA. |
Molecular Mass : | The recombinant human IL12B consists of 317 amino acids and has a predicted molecular mass of 36.2 kDa. As a result of glycosylation, the apparent molecular mass of rhIL12B is approximately 40-50 kDa in SDS-PAGE under reducing conditions. |
Endotoxin : | < 1.0 EU per μg of the protein as determined by the LAL method |
Purity : | > 95 % as determined by SDS-PAGE |
Storage : | Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
Reconstitution : | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |
Gene Name : | IL12B interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40) [ Homo sapiens ] |
Official Symbol : | IL12B |
Synonyms : | IL12B; interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40); NKSF2; interleukin-12 subunit beta; CLMF; CLMF2; cytotoxic lymphocyte maturation factor 2; p40; IL 12B; IL12; subunit p40; interleukin 12; interleukin 12 beta chain; natural killer cell stimulatory factor; 40 kD subunit; natural killer cell stimulatory factor 2; NKSF; CLMF p40; IL-12 subunit p40; IL12, subunit p40; interleukin 12, p40; interleukin-12 beta chain; NK cell stimulatory factor chain 2; cytotoxic lymphocyte maturation factor 40 kDa subunit; natural killer cell stimulatory factor, 40 kD subunit; IL-12B; |
Gene ID : | 3593 |
mRNA Refseq : | NM_002187 |
Protein Refseq : | NP_002178 |
MIM : | 161561 |
UniProt ID : | P29460 |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Customer Reviews (3)
Write a reviewGood for cytokine assays.
Effective in Th2 cell differentiation.
High yield in expression.
Q&As (10)
Ask a questionChIP-seq identifies regulatory elements controlling IL12B gene expression.
Reporter assays monitor IL12B promoter activity under varying conditions.
Primary cell stimulation assays gauge IL12B response to pathogens.
siRNA knockdown uncovers IL12B's role in immune cell activation pathways.
Recombinant protein expression enables functional IL12B studies in vitro.
Co-IP validates IL12B's binding to specific signaling partners.
Genetic variant analysis reveals impacts on IL12B protein function.
Flow cytometry profiles IL12B expression across diverse immune cell types.
Cytokine secretion assays measure IL12B's response kinetics to stimuli.
Co-IP and immunoprecipitation assess IL12B's interactions with other proteins.
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