Recombinant Human IRS1 HEK293T cell lysate
Cat.No. : | IRS1-12HCL |
Product Overview : | Human IRS1 was derived in Human HEK293T cell line. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. |
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Description : | This gene encodes a protein which is phosphorylated by insulin receptor tyrosine kinase. Mutations in this gene are associated with type II diabetes and susceptibility to insulin resistance. |
Source : | HEK293 |
Species : | Human |
Tag : | Myc&DDK |
Form : | The cell lysate is provided in RIPA buffer. |
Molecular Mass : | 131.4 kDa |
Storage : | Upon receiving, store the sample at -20 centigrade. Lysate samples are stable for 12 months from date of receipt when stored at -20 centigrade. Avoid repeated freeze-thaw cycles.Lysate samples can be diluted with 2xSDS Sample Buffer provided. After dilution, the protein sample should be aliquoted and stored at -20 centigrade for long term storage. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes. |
Gene Name : | IRS1 insulin receptor substrate 1 [ Homo sapiens ] |
Official Symbol : | IRS1 |
Synonyms : | IRS1; insulin receptor substrate 1; HIRS 1; IRS-1; HIRS-1; |
Gene ID : | 3667 |
mRNA Refseq : | NM_005544 |
Protein Refseq : | NP_005535 |
MIM : | 147545 |
UniProt ID : | P35568 |
Chromosome Location : | 2q36 |
Products Types
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IRS1-2338H | Recombinant Human IRS1 Protein, His-tagged | +Inquiry |
IRS1-2738H | Recombinant Human IRS1 protein(831-900 aa), C-His-tagged | +Inquiry |
IRS1-4610M | Recombinant Mouse IRS1 Protein, His (Fc)-Avi-tagged | +Inquiry |
◆ Lysates | ||
IRS1-872HCL | Recombinant Human IRS1 cell lysate | +Inquiry |
Related Gene
Antibody levels remain high to one-year’s follow-up after moderate and severe COVID-19, but not after mild cases
Journal: Infectious Diseases (London, England) PubMed ID: 34951554 Data: 2021/12/24
Authors: Anne Kallaste, Kalle Kisand, Margus Lember
Article Snippet:SARS-CoV-2 S RBD (aa 329–538) fragment was cloned into a pNanoLuc vector, and LIPS was performed as reported [ , ].SARS-CoV-2 S RBD (aa 329–538) fragment was cloned into a pNanoLuc vector, and LIPS was performed as reported [ , ].. The transfected HEK293 cell supernatants containing NanoLuc‐fusion protein (10 6 luminescence units; LU) were incubated with serum samples (in triplicate) and Protein G Sepharose beads (Creative BioMart) to capture antibodies.. After washing, the substrate was added (Nano-Glo? Luciferase Substrate, Promega), and luminescence was measured in VICTOR X Reader (PerkinElmer Life Sciences).After washing, the substrate was added (Nano-Glo? Luciferase Substrate, Promega), and luminescence was measured in VICTOR X Reader (PerkinElmer Life Sciences).
Not For Human Consumption!
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