Recombinant Human IRS1 HEK293T cell lysate

• Cat.No.: IRS1-12HCL
• Product Overview: Human IRS1 was derived in Human HEK293T cell line. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate.

Specification

• Species: Human
• Source: HEK293
• Tag: DDK&Myc
• Description: This gene encodes a protein which is phosphorylated by insulin receptor tyrosine kinase. Mutations in this gene are associated with type II diabetes and susceptibility to insulin resistance.
• Form: The cell lysate is provided in RIPA buffer.
• Molecular Mass: 131.4 kDa
• Storage: Upon receiving, store the sample at -20 centigrade. Lysate samples are stable for 12 months from date of receipt when stored at -20 centigrade. Avoid repeated freeze-thaw cycles.Lysate samples can be diluted with 2xSDS Sample Buffer provided. After dilution, the protein sample should be aliquoted and stored at -20 centigrade for long term storage. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.

Gene Information

• Gene Name: IRS1 insulin receptor substrate 1 [ Homo sapiens ]
• Official Symbol: IRS1
• Synonyms: IRS1; insulin receptor substrate 1; HIRS 1; IRS-1; HIRS-1
• Gene ID: 3667
• mRNA Refseq: NM_005544
• Protein Refseq: NP_005535
• MIM: 147545
• UniProt ID: P35568
• Chromosome Location: 2q36

Citation

Antibody levels remain high to one-year’s follow-up after moderate and severe COVID-19, but not after mild cases

Journal: Infectious Diseases (London, England)    PubMed ID: 34951554    Data: 2021/12/24

Authors: Anne Kallaste, Kalle Kisand, Margus Lember

Article Snippet:SARS-CoV-2 S RBD (aa 329–538) fragment was cloned into a pNanoLuc vector, and LIPS was performed as reported [ , ].SARS-CoV-2 S RBD (aa 329–538) fragment was cloned into a pNanoLuc vector, and LIPS was performed as reported [ , ].. The transfected HEK293 cell supernatants containing NanoLuc‐fusion protein (10 6 luminescence units; LU) were incubated with serum samples (in triplicate) and Protein G Sepharose beads (Creative BioMart) to capture antibodies.. After washing, the substrate was added (Nano-Glo? Luciferase Substrate, Promega), and luminescence was measured in VICTOR X Reader (PerkinElmer Life Sciences).After washing, the substrate was added (Nano-Glo? Luciferase Substrate, Promega), and luminescence was measured in VICTOR X Reader (PerkinElmer Life Sciences).